...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ciona intestinalis Hox gene cluster: Its dispersed structure and residual colinear expression in development
【24h】

Ciona intestinalis Hox gene cluster: Its dispersed structure and residual colinear expression in development

机译：Ciona intestinalis Hox基因簇：其分散的结构和在发育中的残留共线性表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ascidians, belonging to the subphylum Urochordata, the earliest branch from the lineage to the vertebrates, exhibit a prototypical morphogenesis of chordates in the larval development, although they subsequently metamorphose into adults with a unique body structure. Recent draft genome analysis of the ascidian Ciona intestinalis has identified 9 Hox genes, which, however, have been located on five scaffolds. Similarly, expression patterns of Ciona Hox genes are largely unknown, although some data have been available for a few Hox member genes. Thus, the cluster structure and colinearity of Hox genes are still an enigma in C. intestinalis. To address these issues, we used fluorescence in situ hybridization and whole-mount in situ hybridization techniques and examined the genomic organization and spatiotemporal expression of all Hox as well as extended Hox member genes (Evx and Mox) of C. intestinalis. We found that seven of nine Ciona Hox genes are located on a single chromosome with some ordering exceptions, and the other genes, including Evx and Mox, are located on three other chromosomes. Some Ciona Hox genes, if not all, exhibited spatially coordinated expression within the larval central nervous system and the gut of the juvenile. In light of these observations, we suggest that the cluster organization and colinearity of the Hox genes are under dispersing and disintegrating conditions in C. intestinalis.
机译：属于Urochordata亚科（从血统到脊椎动物最早的分支）的海鞘显示出幼虫发育过程中脊索动物的原型形态发生，尽管随后它们变成具有独特身体结构的成虫。最近对海鞘Ciona intestinalis进行的基因组分析草案确定了9个Hox基因，但是它们位于五个支架上。类似地，尽管有一些Hox成员基因的可用数据，但Ciona Hox基因的表达模式基本上是未知的。因此，Hox基因的簇结构和共线性在小肠念珠菌中仍然是一个谜。为了解决这些问题，我们使用了荧光原位杂交和整装原位杂交技术，并检查了肠球菌的所有Hox以及扩展的Hox成员基因（Evx和Mox）的基因组组织和时空表达。我们发现九个Ciona Hox基因中的七个位于单个染色体上，但有一些有序例外，而其他基因（包括Evx和Mox）位于其他三个染色体上。某些Ciona Hox基因（如果不是全部的话）在幼虫中枢神经系统和幼体的肠道内表现出空间协调的表达。根据这些观察结果，我们建议Hox基因的簇组织和共线性处于肠小肠弯曲菌的分散和崩解条件下。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号