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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The contribution of epistasis to the architecture of fitness in an RNA virus
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The contribution of epistasis to the architecture of fitness in an RNA virus

机译：上位性对RNA病毒适应性结构的贡献

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The tendency for genetic architectures to exhibit epistasis among mutations plays a central role in the modern synthesis of evolutionary biology and in theoretical descriptions of many evolutionary processes. Nevertheless, few studies unquestionably show whether, and how, mutations typically interact. Beneficial mutations are especially difficult to identify because of their scarcity. Consequently, epistasis among pairs of this important class of mutations has, to our knowledge, never before been explored. Interactions among genome components should be of special relevance in compacted genomes such as those of RNA viruses. To tackle these issues, we first generated 47 genotypes of vesicular stomatitis virus carrying pairs of nucleotide substitution mutations whose separated and combined deleterious effects on fitness were determined. Several pairs exhibited significant interactions for fitness, including antagonistic and synergistic epistasis. Synthetic lethals represented 50% of the latter. In a second set of experiments, 15 genotypes carrying pairs of beneficial mutations were also created. In this case, all significant interactions were antagonistic. Our results show that the architecture of the fitness depends on complex interactions among genome components.
机译：遗传结构在突变中表现出上位性的趋势在进化生物学的现代合成和许多进化过程的理论描述中起着核心作用。然而，很少有研究无疑显示出突变是否以及如何相互作用。有益突变由于其稀缺性而特别难以鉴定。因此，就我们所知，这种重要的突变类别之间的上位性从未被探索过。基因组成分之间的相互作用在紧密的基因组（如RNA病毒）中应特别相关。为了解决这些问题，我们首先产生了47种带有一对核苷酸取代突变的水疱性口炎病毒基因型，这些突变对已确定了对健康的分离和综合有害作用。几对显示出适合健身的重要相互作用，包括拮抗和协同上位。合成杀伤力占后者的50％。在第二组实验中，还创建了15对携带有益突变对的基因型。在这种情况下，所有重要的相互作用都是拮抗的。我们的结果表明，适应度的体系结构取决于基因组组件之间的复杂相互作用。

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