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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA
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Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA

机译：Imp3p和Imp4p介导必需的U3-前体rRNA（pre-rRNA）双链体的形成，可能是为了将小的亚基过程组募集到pre-rRNA

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摘要

In eukaryotes, formation of short duplexes between the U3 small nucleolar RNA (snoRNA) and the precursor rRNA (pre-rRNA) at multiple sites is a prerequisite for three endonucleolytic cleavages that initiate small subunit biogenesis by releasing the 18S rRNA precursor from the pre-rRNA. The most likely role of these RNA duplexes is to guide the U3 snoRNA and its associated proteins, designated the small subunit processome, to the target cleavage sites on the pre-rRNA. Studies by others in Saccharomyces cerevisiae have identified the proteins Mpp10p, Imp3p, and Imp4p as candidates to mediate U3-pre-rRNA interactions. We report here that Imp3p and Imp4p appear to stabilize an otherwise unstable duplex between the U3 snoRNA hinge region and complementary bases in the external transcribed spacer of the pre-rRNA. In addition, Imp4p, but not Imp3p, seems to rearrange the U3 box A stem structure to expose the site that base-pairs with the 5' end of the 18S rRNA, thereby mediating duplex formation at a second site. By mediating formation of both essential U3-pre-rRNA duplexes, Imp3p and Imp4p may help the small subunit processome to dock onto the pre-rRNA, an event indispensable for ribosome biogenesis and hence for cell growth.
机译：在真核生物中，U3小核仁RNA（snoRNA）和前体rRNA（pre-rRNA）在多个位点之间形成短双链体是三个内切核酸酶裂解的先决条件，这些裂解通过从前体释放18S rRNA前体来启动小亚基生物发生。 rRNA。这些RNA双链体最可能的作用是将U3 snoRNA及其相关蛋白（称为小亚基加工组）引导至pre-rRNA上的目标切割位点。酿酒酵母（Saccharomyces cerevisiae）中其他人的研究已经确定了蛋白Mpp10p，Imp3p和Imp4p是介导U3-pre-rRNA相互作用的候选蛋白。我们在这里报告，Imp3p和Imp4p似乎稳定了U3 snoRNA铰链区与pre-rRNA的外部转录间隔区中的互补碱基之间的否则不稳定的双链体。此外，Imp4p，而不是Imp3p，似乎重新排列了U3 box A茎结构，以暴露与18S rRNA 5'端碱基配对的位点，从而介导了第二个位点的双链体形成。通过介导两个必需的U3-pre-rRNA双链体的形成，Imp3p和Imp4p可以帮助小的亚基过程组停靠在pre-rRNA上，这是核糖体生物发生以及细胞生长所不可缺少的事件。

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