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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome.
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Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome.

机译：由20 kb病毒RNA基因组编码的三种不同的RNA沉默抑制子。

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摘要

Viral infection in both plant and invertebrate hosts requires a virus-encoded function to block the RNA silencing antiviral defense. Here, we report the identification and characterization of three distinct suppressors of RNA silencing encoded by the approximately 20-kb plus-strand RNA genome of citrus tristeza virus (CTV). When introduced by genetic crosses into plants carrying a silencing transgene, both p20 and p23, but not coat protein (CP), restored expression of the transgene. Although none of the CTV proteins prevented DNA methylation of the transgene, export of the silencing signal (capable of mediating intercellular silencing spread) was detected only from the F(1) plants expressing p23 and not from the CP- or p20-expressing F(1) plants, demonstrating suppression of intercellular silencing by CP and p20 but not by p23. Thus, intracellular and intercellular silencing are each targeted by a CTV protein, whereas the third, p20, inhibits silencing at both levels. Notably, CP suppresses intercellular silencing without interfering with intracellular silencing. The novel property of CP suggests a mechanism distinct to p20 and all of the other viral suppressors known to interfere with intercellular silencing and that this class of viral suppressors may not be consistently identified by Agrobacterium coinfiltration because it also induces RNA silencing against the infiltrated suppressor transgene. Our analyses reveal a sophisticated viral counter-defense strategy that targets the silencing antiviral pathway at multiple steps and may be essential for protecting CTV with such a large RNA genome from antiviral silencing in the perennial tree host.
机译：植物和无脊椎动物宿主中的病毒感染都需要一种病毒编码的功能来阻断RNA沉默抗病毒防御。在这里，我们报告鉴定和表征的柑橘沉默的三链带状病毒（CTV）的大约20 kb的正链RNA基因组编码的RNA沉默的三个不同抑制器。当通过遗传杂交导入携带沉默转基因的植物时，p20和p23均未恢复外壳蛋白（CP），但恢复了转基因的表达。虽然没有CTV蛋白阻止转基因的DNA甲基化，但沉默信号的输出（能够介导细胞间沉默传播）仅从表达p23的F（1）植物中检测到，而从表达CP-或p20的F（ 1）植物，表明CP和p20抑制了细胞间的沉默，而p23则没有。因此，细胞内和细胞间沉默均被CTV蛋白靶向，而第三个蛋白p20在两个水平上均抑制沉默。值得注意的是，CP抑制细胞间沉默而不干扰细胞内沉默。 CP的新颖特性表明，它与p20和所有其他已知能干扰细胞间沉默的病毒抑制剂不同，该机制可能不被农杆菌共滤法一致地鉴定，因为它还诱导了针对渗透抑制基因的RNA沉默。 。我们的分析揭示了一种复杂的病毒防御策略，该策略针对多个步骤沉默沉默的抗病毒途径，对于保护具有如此大RNA基因组的CTV免受多年生树木宿主中的抗病毒沉默可能是必不可少的。

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