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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Proteins of purified Epstein-Barr virus.
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Proteins of purified Epstein-Barr virus.

机译：纯化的爱泼斯坦-巴尔病毒的蛋白质。

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摘要

Mature Epstein-Barr virus (EBV) was purified from the culture medium of infected lymphocytes made functionally conditional for Zta activation of lytic replication by an in-frame fusion with a mutant estrogen receptor. Proteins in purified virus preparations were separated by gradient gel electrophoresis and trypsin-digested; peptides were then analyzed by tandem hydrophobic chromatography, tandem MS sequencing, and MS scans. Potential peptides were matched with EBV and human gene ORFs. Mature EBV was mostly composed of homologues of proteins previously found in a herpes virion. However, EBV homologues to herpes simplex virus capsid-associated or tegument components UL7 (BBRF2), UL14 (BGLF3), and EBV BFRF1 were not significantly detected. Instead, probable tegument components included the EBV and gamma-herpesvirus-encoded BLRF2, BRRF2, BDLF2 and BKRF4 proteins. Actin was also a major tegument protein, and cofilin, tubulin, heat shock protein 90, and heat shock protein 70 were substantial components. EBVenvelope glycoprotein gp350 was highly abundant, followed by glycoprotein gH, intact and furin-cleaved gB, gM, gp42, gL, gp78, gp150, and gN. BILF1 (gp64) and proteins associated with latent EBV infection were not detected in virions.
机译：从感染的淋巴细胞的培养基中纯化成熟的爱泼斯坦-巴尔病毒（EBV），该淋巴细胞的功能性条件是通过与突变雌激素受体的框内融合来实现裂解复制的Zta激活。纯化的病毒制品中的蛋白质通过梯度凝胶电泳分离并用胰蛋白酶消化；然后通过串联疏水色谱，串联MS测序和MS扫描对多肽进行分析。潜在的肽与EBV和人类基因ORF匹配。成熟的EBV主要由先前在疱疹病毒粒子中发现的蛋白质的同源物组成。但是，未明显检测到与单纯疱疹病毒衣壳相关或外皮成分UL7（BBRF2），UL14（BGLF3）和EBV BFRF1的EBV同源物。相反，可能的外皮成分包括EBV和伽马疱疹病毒编码的BLRF2，BRRF2，BDLF2和BKRF4蛋白。肌动蛋白也是主要的被膜蛋白，而cofilin，微管蛋白，热休克蛋白90和热休克蛋白70是主要成分。 EBVenvelope糖蛋白gp350非常丰富，其次是糖蛋白gH，完整和弗林蛋白酶切割的gB，gM，gp42，gL，gp78，gp150和gN。在病毒体中未检测到BILF1（gp64）和与潜在EBV感染相关的蛋白质。

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