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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >RecA-dependent mutants in Escherichia coli reveal strategies to avoid chromosomal fragmentation.
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RecA-dependent mutants in Escherichia coli reveal strategies to avoid chromosomal fragmentation.

机译：大肠杆菌中RecA依赖性突变体揭示了避免染色体片段化的策略。

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摘要

RecA- and RecBC-catalyzed repair in eubacteria assembles chromosomes fragmented by double-strand breaks. We propose that recA mutants, being unable to repair fragmented chromosomes, depend on various strategies designed to avoid chromosomal fragmentation. To identify chromosomal fragmentation-avoidance strategies, we screened for Escherichia coli mutants synthetically inhibited in combination with recA inactivation by identifying clones unable to lose a plasmid carrying the recA(+) gene. Using this screen, we have isolated several RecA-dependent mutants and assigned them to three distinct areas of metabolism. The tdk and rdgB mutants affect synthesis of DNA precursors. The fur, ubiE, and ubiH mutants are likely to have increased levels of reactive oxygen species. The seqA, topA mutants and an insertion in smtA perturbing the downstream mukFEB genes affect nucleoid administration. All isolated mutants show varying degree of SOS induction, indicating elevated levels of chromosomal lesions. As predicted, mutants in rdgB, seqA, smtA, topA, and fur show increased levels of chromosomal fragmentation in recBC mutant conditions. Future characterization of these RecA-dependent mutants will define mechanisms of chromosomal fragmentation avoidance.
机译：在真细菌中，RecA和RecBC催化的修复可组装由双链断裂片段化的染色体。我们建议recA突变体，无法修复染色体碎片，取决于设计避免染色体片段化的各种策略。为了确定避免染色体断裂的策略，我们通过鉴定无法丢失携带recA（+）基因的质粒的克隆，筛选了与recA失活联合抑制的大肠杆菌突变体。使用此筛选，我们分离了几个RecA依赖性突变体，并将它们分配给了三个不同的代谢区域。 tdk和rdgB突变体影响DNA前体的合成。 fur，ubiE和ubiH突变体可能具有增加的活性氧水平。 seqA，topA突变体和干扰下游mukFEB基因的smtA插入会影响类核苷酸的给药。所有分离的突变体均表现出不同程度的SOS诱导，表明染色体病变水平升高。如预期的那样，在recBC突变条件下，rdgB，seqA，smtA，topA和fur中的突变体显示出增加的染色体片段水平。这些RecA依赖突变体的未来表征将定义避免染色体片段化的机制。

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