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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cell-surface expression of a mutated Epstein-Barr virus glycoprotein B allows fusion independent of other viral proteins.
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Cell-surface expression of a mutated Epstein-Barr virus glycoprotein B allows fusion independent of other viral proteins.

机译：突变的爱泼斯坦-巴尔病毒糖蛋白B在细胞表面的表达可使其融合独立于其他病毒蛋白。

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摘要

Epstein-Barr virus (EBV) infects human B lymphocytes and epithelial cells. We have compared the requirements for EBV glycoprotein-induced cell fusion between Chinese hamster ovary effecter cells and human B lymphoblasts or epithelial cells by using a virus-free cell fusion assay. EBV-encoded gB, gH, gL, and gp42 glycoproteins were required for efficient B cell fusion, whereas EBV gB, gH, and gL glycoproteins were required for Chinese hamster ovary effecter cell fusion with epithelial cell lines (AGS and SCC68) or the human embryonic kidney cell line 293-P. Fusion with human embryonic kidney 293-P cells was greater than fusion observed with B cells, indicative of an important role for cell contact. An antibody directed against the gH and gL complex inhibited epithelial cell fusion. Increased surface expression of gB alone as a result of truncations or point mutants in the carboxyl-terminal tail allowed gB-mediated fusion with epithelial cells, albeit at a lower level than with coexpression of gB, gH, and gL. Overall, gB appears to be the critical component for EBV glycoprotein-mediated cell fusion.
机译：爱泼斯坦巴尔病毒（EBV）感染人类B淋巴细胞和上皮细胞。我们通过使用无病毒细胞融合测定法，比较了中国仓鼠卵巢效应细胞与人B淋巴母细胞或上皮细胞之间EBV糖蛋白诱导的细胞融合的要求。有效的B细胞融合需要EBV编码的gB，gH，gL和gp42糖蛋白，而中国仓鼠卵巢效应细胞与上皮细胞系（AGS和SCC68）或人的融合需要EBV gB，gH和gL糖蛋白胚胎肾细胞系293-P。与人类胚胎肾293-P细胞的融合大于与B细胞的融合，这表明细胞接触具有重要作用。针对gH和gL复合物的抗体抑制上皮细胞融合。由于截短或羧基末端尾巴中的点突变，单独增加了gB的表面表达，使gB介导的与上皮细胞融合，尽管其水平低于与gB，gH和gL的共表达水平。总体而言，gB似乎是EBV糖蛋白介导的细胞融合的关键成分。

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