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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Vesicular re uptake inhibition by a synaptotagmin I C2B domain antibody at the squid giant synapse
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Vesicular re uptake inhibition by a synaptotagmin I C2B domain antibody at the squid giant synapse

机译：鱿鱼巨突触中突触标记蛋白I C2B域抗体对水泡再摄取的抑制作用

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摘要

Synaptotagmin (Syt) I, a ubiquitous synaptic vesicle protein, comprises a transmembrane region and two C2 domains. The C2 domains, which have been shown to be essential for both synaptic vesicle exocytosis and endocytosis, are also seen as the Ca~(2+) sensors in synaptic vesicular release. In a previous study, we reported that a polyclonal antibody raised against the squid (Loligo pealei) Syt I C2B domain, while inhibiting vesicular endocytosis, was synaptic release neutral at the squid giant synapse. Recent reports concerning the C2B requirements for synaptic release prompted us to readdress the rote of C2B in squid giant synapse function. Presynaptic injection of another anti-Syt I-C2B antibody (using recombinant whole C2B domain expressed in mammalian cell culture as an antigen) into the presynaptic terminal reproduced our previous results, i.e., reduction of vesicular endocytosis without affecting synaptic release. This set of results addresses the issue of the geometrical arrangement of the Ca~(2+) sensor, allowing the C2B domain antibody to restrict CCa~(2+)-dependent C2B self-oligomerization without modifying the Ca~(2+)-dependent release process.
机译：突触标签蛋白（Syt）I（一种普遍存在的突触小泡蛋白）包含一个跨膜区和两个C2域。 C2结构域已被证明对于突触囊泡胞吐作用和胞吞作用都是必不可少的，也被视为突触囊泡释放中的Ca〜（2+）传感器。在先前的研究中，我们报道了针对鱿鱼（Loligo pealei）Syt I C2B域的多克隆抗体，虽然抑制了囊泡的内吞作用，但在鱿鱼巨突触中突触释放为中性。有关突触释放的C2B要求的最新报道促使我们重新讨论鱿鱼巨型突触功能中C2B的死记硬背。突触前向突触前末端注射另一种抗Syt I-C2B抗体（使用在哺乳动物细胞培养物中表达的重组完整C2B结构域作为抗原）重现了我们以前的结果，即减少囊泡内吞作用而不影响突触释放。这组结果解决了Ca〜（2+）传感器的几何排列问题，允许C2B域抗体在不修饰Ca〜（2 +）-的情况下限制CCa〜（2+）依赖性C2B自寡聚。依赖发布过程。

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