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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds
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Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds

机译:通过电刺激支架上培养的心肌细胞对工程化心肌进行功能组装

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摘要

The major challenge of tissue engineering is directing the cells to establish the physiological structure and function of the tissue being replaced across different hierarchical scales. To engineer myocardium, biophysical regulation of the cells needs to recapitulate multiple signals present in the native heart. We hypothesized that excitation-contraction coupling, critical for the development and function of a normal heart, determines the development and function of engineered myocardium. To induce synchronous contractions of cultured cardiac constructs, we applied electrical signals designed to mimic those in the native heart. Over only 8 days in vitro, electrical field stimulation induced cell alignment and coupling, increased the amplitude of synchronous construct contractions by a factor of 7, and resulted in a remarkable level of ultrastructural organization. Development of conductive and contractile properties of cardiac constructs was concurrent, with strong dependence on the initiation and duration of electrical stimulation.
机译:组织工程学的主要挑战是指导细胞建立跨不同等级尺度被替换的组织的生理结构和功能。为了改造心肌,细胞的生物物理调节需要概括天然心脏中存在的多种信号。我们假设,对于正常心脏的发育和功能至关重要的激发-收缩耦合决定了工程心肌的发育和功能。为了诱导培养的心脏构造物的同步收缩,我们应用了旨在模拟天然心脏中的信号的电信号。在体外仅8天的时间里,电场刺激即可诱导细胞排列和偶联,使同步构建体的收缩幅度增加7倍,并导致超微结构的显着水平。心脏结构的传导性和收缩性的发展是同时发生的,强烈依赖于电刺激的开始和持续时间。

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