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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM. Atomic force microscopy.
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Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM. Atomic force microscopy.

机译：AFM在天然膜中对绿假单胞菌光合核心复合体进行纳米解剖和高分辨率成像。原子力显微镜。

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摘要

In photosynthesis, highly organized multiprotein assemblies convert sunlight into biochemical energy with high efficiency. A challenge in structural biology is to analyze such supramolecular complexes in native membranes. Atomic force microscopy (AFM) with high lateral resolution, high signal-to-noise ratio, and the possibility to nanodissect biological samples is a unique tool to investigate multiprotein complexes at molecular resolution in situ. Here we present high-resolution AFM of the photosynthetic core complex in native Rhodopseudomonas viridis membranes. Topographs at 10-A lateral and approximately 1-A vertical resolution reveal a single reaction center (RC) surrounded by a closed ellipsoid of 16 light-harvesting (LH1) subunits. Nanodissection of the tetraheme cytochrome (4Hcyt) subunit from the RC allows demonstration that the L and M subunits exhibit an asymmetric topography intimately associated to the LH1 subunits located at the short ellipsis axis. This architecture implies a distance distribution between the antenna and the RC compared with a centered location of the RC within a circular LH1, which may influence the energy transfer within the core complex. The LH1 subunits rearrange into a circle after removal of the RC from the core complex.
机译：在光合作用中，高度组织化的多蛋白装配将太阳光高效转化为生化能。结构生物学的挑战是分析天然膜中的这种超分子复合物。具有高横向分辨率，高信噪比的原子力显微镜（AFM），以及对生物样品进行纳米解剖的可能性，是一种以分子分辨率就地研究多蛋白复合物的独特工具。在这里，我们介绍了天然红假单胞菌膜的光合核心复合体的高分辨率AFM。横向分辨率为10A且垂直分辨率约为1A的地形图显示，单个反应中心（RC）被16个光收集（LH1）子单元的封闭椭球包围。从RC的四血红素细胞色素（4Hcyt）亚基的纳米剖析可以证明L和M亚基表现出与位于短椭圆轴的LH1亚基紧密相关的不对称形貌。与圆形LH1中RC的居中位置相比，这种架构意味着天线与RC之间的距离分布，这可能会影响核心复合体内的能量传输。从核心复合物中除去RC后，LH1亚基重排成一个圆圈。

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