...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Chemosensory regulation of developmental gene expression in Myxococcusxanthus.
【24h】

Chemosensory regulation of developmental gene expression in Myxococcusxanthus.

机译：粘球菌中发育基因表达的化学感应调控。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The delta-proteobacterium Myxococcus xanthus coordinates its motility during aggregation and fruiting body formation. While searching for chemotaxis genes in M. xanthus, we identified a third chemotaxis-like gene cluster, the che3 cluster, encoding homologs to two methyl-accepting chemotaxis proteins (MCPs), a CheW, a hybrid CheA, a CheB, a CheR, but no CheY. Mutations in mcp3A, mcp3B, and cheA3 did not show obvious defects in motility or chemotaxis but did affect the timing of entry into development. Mutations in these genes caused early aggregation of starving cells, even at low cell densities. Furthermore, these mutants showed pronounced overexpression of the developmentally regulated Tn5lac fusions Omega4403, Omega4411, and Omega4521 as well as overexpression of mbhA and tps, markers for peripheral rods and aggregating cells, respectively. Divergently transcribed from the che3 promoter region is another gene, crdA (chemosensory regulator of development), predicted to encode a transcriptional activator of varsigma(54)-dependent promoters. To test the hypothesis that CrdA functions as the cognate response regulator for the histidine kinase CheA3, CrdA and CheA3 were assayed and found to interact strongly in the yeast two-hybrid system. Mutant analysis showed that crdA cells were delayed in development (12-24 h) and delayed in MbhA production relative to the wild type. An mcp3BcrdA double mutant displayed the crdA phenotype, indicating that crdA is epistatic to mcp3B. We conclude that the Che3 chemotaxis-like system functions to control developmental gene expression by regulating a varsigma(54) transcriptional activator, CrdA.
机译：三角洲变形杆菌（Myxococcus xanthus）在聚集和子实体形成期间协调其运动。在寻找黄腐分支杆菌的趋化性基因时，我们发现了第三个趋化性样基因簇，即che3簇，编码两个甲基接受趋化蛋白（MCP），CheW，杂化CheA，CheB，CheR，但是没有Chey。 mcp3A，mcp3B和cheA3中的突变并未显示出运动性或趋化性的明显缺陷，但确实影响了进入发育的时间。这些基因的突变导致饥饿的细胞尽早聚集，即使在低细胞密度下也是如此。此外，这些突变体显示出发育受调节的Tn5lac融合蛋白Omega4403，Omega4411和Omega4521的过表达，以及mbhA和tps的过表达，它们分别是外周杆和聚集细胞的标志。从che3启动子区域不同地转录的是另一个基因crdA（发育的化学感觉调节剂），预计可编码varsigma（54）依赖性启动子的转录激活因子。为了检验关于CrdA充当组氨酸激酶CheA3的同源响应调节剂的假设，对CrdA和CheA3进行了分析，发现它们在酵母双杂交系统中有很强的相互作用。突变分析表明，相对于野生型，crdA细胞发育延迟（12-24小时），MbhA生产延迟。一个mcp3BcrdA双重突变体显示crdA表型，表明crdA对mcp3B上位。我们的结论是，Che3趋化性样系统的功能是通过调节varsigma（54）转录激活因子CrdA来控制发育基因的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号