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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >DNA unzipped under a constant force exhibits multiple metastable intermediates.
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DNA unzipped under a constant force exhibits multiple metastable intermediates.

机译：在恒力作用下解压缩的DNA表现出多种亚稳中间体。

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摘要

Single molecule studies, at constant force, of the separation of double-stranded DNA into two separated single strands may provide information relevant to the dynamics of DNA replication. At constant applied force, theory predicts that the unzipped length as a function of time is characterized by jumps during which the strands separate rapidly, followed by long pauses where the number of separated base pairs remains constant. Here, we report previously uncharacterized observations of this striking behavior carried out on a number of identical single molecules simultaneously. When several single lambda phage molecules are subject to the same applied force, the pause positions are reproducible in each. This reproducibility shows that the positions and durations of the pauses in unzipping provide a sequence-dependent molecular fingerprint. For small forces, the DNA remains in a partially unzipped state for at least several hours. For larger forces, the separation is still characterized by jumps and pauses, but the double-stranded DNA will completely unzip in less than 30 min.
机译：将双链DNA分离成两条分离的单链的恒力单分子研究可能会提供与DNA复制动力学有关的信息。在恒定的作用力下，理论预测，随时间变化的解压缩长度的特征在于跳跃，在跳跃过程中，链快速分离，随后是长时间的停顿，其中分离的碱基对的数量保持恒定。在这里，我们报告以前在多个相同的单个分子上同时执行此惊人行为的特征观察。当几个单个λ噬菌体分子受到相同的作用力时，每个位置都可以重现。这种可再现性表明，解压缩过程中暂停的位置和持续时间提供了序列依赖性分子指纹。对于较小的作用力，DNA至少在几个小时内保持部分解压缩状态。对于较大的作用力，分离仍然具有跳跃和停顿的特征，但是双链DNA将在不到30分钟的时间内完全解压缩。

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