...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Requirement for Pangolin/dTCF in Drosophila Wingless signaling
【24h】

Requirement for Pangolin/dTCF in Drosophila Wingless signaling

机译：果蝇无翅信号传递对穿山甲/ dTCF的要求

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Wingless (Wg) protein is a secreted glycoprotein involved in intercellular signaling. On activation of the Wg signaling pathway. Armadillo is stabilized, causing target genes to be activated by the transcription factor Pangolin (Pan). This study investigated the roles of Pan in the developing wing of Drosophila by clonal analysis. Three different aspects of wing development were examined: cell proliferation, wing margin specification, and wg self-refinement. Our results indicate that Pan function is critically required for all three of these processes. Consequently, lack of pan causes a severe reduction in the activity of the Wg target genes Distalless and vestigial within their normal domain of expression. Loss of pan function does not, however, lead to a derepression of these genes outside this domain. Thus, although Pan is positively required for the induction of Wg targets in the wing imaginal disk, it does not appear to play a default repressor function in the absence of Wg input. In contrast, lack of zygotic pan function causes a milder phenotype than that caused by the lack of wg function in the embryo. We show that this difference cannot be attributed to maternally provided pan product, indicating that a Pan represser function usually prevents the expression of embryonic Wg targets. Together, our results suggest that for embryonic patterning the activator as well as represser forms of Pan play important roles, while for wing development Pan operates primarily in the activator mode.
机译：无翅（Wg）蛋白是一种参与细胞间信号传导的分泌糖蛋白。 Wg信号通路的激活。犰狳被稳定化，导致目标基因被转录因子Pangolin（Pan）激活。本研究通过克隆分析研究了潘在果蝇发育中的作用。检查了机翼发育的三个不同方面：细胞增殖，机翼边缘规格和wg自我完善。我们的结果表明，所有这三个过程都非常需要Pan功能。因此，缺乏泛导致Wg靶基因在其正常表达域内无离散和残留的活性严重降低。但是，泛功能的丧失不会导致这些基因在该结构域之外的抑制。因此，尽管在机翼假想盘中诱导Wg目标确实需要Pan，但在没有Wg输入的情况下，它似乎没有发挥默认的阻遏功能。相反，缺乏合子泛功能引起的表型比胚胎中缺乏wg功能引起的表型温和。我们表明，这种差异不能归因于母体提供的泛产品，表明泛阻遏物功能通常会阻止胚胎Wg靶标的表达。总之，我们的结果表明，对于胚胎构图而言，Pan的激活剂和阻遏物形式起着重要的作用，而对于机翼发育，Pan主要在激活剂模式下运行。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号