...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genomewide transcriptional changes associated with genetic alterations and nutritional supplementation affecting tryptophan metabolism in Bacillus subtilis
【24h】

Genomewide transcriptional changes associated with genetic alterations and nutritional supplementation affecting tryptophan metabolism in Bacillus subtilis

机译：全基因组转录变化与影响枯草芽孢杆菌的色氨酸代谢的遗传改变和营养补充有关

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

DNA microarrays comprising ≈95% of the Bacillus subtilis annotated protein coding ORFs were deployed to generate a series of snapshots of genomewide transcriptional changes that occur when cells are grown under various conditions that are expected to increase or decrease transcription of the trp operon segment of the aromatic supraoperon. Comparisons of global expression patterns were made between cells grown in the presence of indole acrylic acid, a specific inhibitor of tRNA~(Trp) charging; cells deficient in expression of the mtrB gene, which encodes the tryptophan-activated negative regulatory protein, TRAP; WT cells grown in the presence or absence of two or three of the aromatic amino acids; and cells harboring a tryptophanyl tRNA synthetase mutation conferring temperature-sensitive tryptophan-dependent growth. Our findings validate expected responses of the tryptophan bio-synthetic genes and presumed regulatory interrelationships between genes in the different aromatic amino acid pathways and the histidine biosynthetic pathway. Using a combination of supervised and unsupervised statistical methods we identified ≈100 genes whose expression profiles were closely correlated with those of the genes in the trp operon. This finding suggests that expression of these genes is influenced directly or indirectly by regulatory events that affect or are a consequence of altered tryptophan metabolism.
机译：部署了包含约95％枯草芽孢杆菌注释蛋白编码ORF的DNA微阵列，以生成一系列全基因组转录变化的快照，这些快照在不同条件下生长细胞时会发生，这些条件预计会增加或减少其trp操纵子片段的转录。芳香素。比较在吲哚丙烯酸（tRNA〜（Trp）电荷的特异性抑制剂）存在下生长的细胞之间的整体表达模式。缺乏编码色氨酸激活的负调控蛋白TRAP的mtrB基因表达的细胞； WT细胞在存在或不存在两种或三种芳香族氨基酸的情况下生长；以及带有色氨酸tRNA合成酶突变的细胞，这些突变赋予温度敏感的色氨酸依赖性生长。我们的发现验证了色氨酸生物合成基因的预期反应以及不同芳香族氨基酸途径和组氨酸生物合成途径中基因之间的推测调控关系。使用有监督和无监督统计方法的组合，我们确定了≈100个基因，它们的表达谱与trp操纵子中的表达谱密切相关。该发现表明，这些基因的表达直接或间接地受到调节事件的影响，所述调节事件影响或是色氨酸代谢改变的结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号