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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana
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Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana

机译：海藻6-磷酸对于拟南芥中碳水化合物的利用和生长必不可少

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摘要

Genes for trehalose metabolism are widespread in higher plants. Insight into the physiological role of the trehalose pathway outside of resurrection plant species is lacking. To address this lack of insight, we express Escherichia coli genes for trehalose metabolism in Arabidopsis thaliana, which manipulates trehalose 6-phosphate (T6P) contents in the transgenic plants. Plants expressing otsA [encoding trehalose phosphate synthase (TPS)] accumulate T6P whereas those expressing either otsB [encoding trehalose phosphate phosphatase (TPP)] or treC [encoding trehalose phosphate hydrolase (TPH)] contain low levels of T6P. Expression of treF (encoding trehalase) yields plants with unaltered T6P content and a phenotype not distinguishable from wild type when grown on soil. The marked phenotype obtained of plants accumulating T6P is opposite to that of plants with low T6P levels obtained by expressing either TPP or TPH and consistent with a critical role for T6P in growth and development. Supplied sugar strongly inhibits growth of plants with reduced T6P content and leads to accumulation of respiratory intermediates. Remarkably, sugar improves growth of TPS expressors over wild type, a feat not previously accomplished by manipulation of metabolism. The data indicate that the T6P intermediate of the trehalose pathway controls carbohydrate utilization and thence growth via control of glycolysis in a manner analogous to that in yeast. Furthermore, embryo-lethal A. thaliana tps1 mutants are rescued by expression of E. coli TPS, but not by supply of trehalose, suggesting that T6P control over primary metabolism is indispensable for development. [References: 48]
机译：海藻糖代谢的基因广泛存在于高等植物中。缺乏对在复活植物物种之外的海藻糖途径的生理作用的了解。为了解决这一不足，我们在拟南芥中表达海藻糖代谢的大肠杆菌基因，该基因操纵转基因植物中的海藻糖6-磷酸（T6P）含量。表达otsA [编码海藻糖磷酸合酶（TPS）]的植物积累T6P，而表达otsB [编码海藻糖磷酸磷酸酶（TPP）]或treC [编码海藻糖磷酸水解酶（TPH）]的植物中T6P含量低。 treF（编码海藻糖酶）的表达产生的植物具有不变的T6P含量，并且在土壤上生长时无法与野生型区分开。积累T6P的植物获得的显着表型与通过表达TPP或TPH获得的低T6P水平的植物相反，并且与T6P在生长发育中的关键作用相一致。所提供的糖强烈抑制T6P含量降低的植物生长，并导致呼吸中间产物积聚。显着地，糖比野生型改善了TPS表达子的生长，这是以前无法通过控制代谢实现的壮举。数据表明，海藻糖途径的T6P中间体以与酵母类似的方式通过控制糖酵解作用来控制碳水化合物的利用并因此控制其生长。此外，通过大肠杆菌TPS的表达拯救了具有致命致命性的拟南芥tps1突变体，但通过海藻糖的供应却无法挽救这种突变，这表明T6P对初级代谢的控制对于发育是必不可少的。 [参考：48]

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