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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Immunization with hepatitis C virus-like particles protects mice from recombinant hepatitis C virus-vaccinia infection
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Immunization with hepatitis C virus-like particles protects mice from recombinant hepatitis C virus-vaccinia infection

机译：接种丙型肝炎病毒样颗粒可保护小鼠免受重组丙型肝炎病毒-牛痘感染

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摘要

We have recently demonstrated that immunization with hepatitis C virus-like particles (HCV-LPs) generated in insect cells can elicit both humoral and cellular immune responses in BALB/c mice. Here, we evaluate the immunogenicity of HCV-LPs in HLA2.1 transgenic (AAD) mice in comparison to DNA immunization. HCV-LP immunization elicited a significantly stronger humoral immune response than DNA immunization. HCV-LP-immunized mice also developed stronger HCV-specific cellular immune responses than DNA-immunized mice as determined by using quantitative enzyme-linked immunospot (ELISpot) assay and intracellular cytokine staining. In BALB/c mice, immunization with HCV-LPs resulted in a >5 log(10) reduction in vaccinia titer when challenged with a recombinant vaccinia expressing the HCV structural proteins (vvHCV.S), as compared to 1 log(10) decrease in DNA immunization. In HLA2.1 transgenic mice, a 1-2 log(10) reduction resulted from HCV-LP immunization, whereas no reduction was seen from DNA immunization. Adoptive transfer of lymphocytes from HCV-LP-immunized mice to naive mice provided protection against vvHCV.S challenge, and this transferred immunity can be abrogated by either CD4 or CD8 depletion. Our results suggest that HCV-LPs can induce humoral and cellular immune responses that are protective in a surrogate HCV challenge model and that a strong cellular immunity provided by both CD4 and CD8 effector lymphocytes may be important for protection from HCV infection. [References: 34]
机译：我们最近证明，用昆虫细胞中产生的丙型肝炎病毒样颗粒（HCV-LPs）进行免疫可以在BALB / c小鼠中引起体液和细胞免疫反应。在这里，我们评估与DNA免疫相比，HLA2.1转基因（AAD）小鼠中HCV-LP的免疫原性。 HCV-LP免疫引起的体液免疫反应比DNA免疫显着强。通过使用定量酶联免疫斑点（ELISpot）分析和细胞内细胞因子染色确定，HCV-LP免疫的小鼠也比DNA免疫的小鼠产生更强的HCV特异性细胞免疫反应。在BALB / c小鼠中，用表达HCV结构蛋白（vvHCV.S）的重组牛痘激发，用HCV-LPs免疫导致牛痘滴度降低> 5 log（10），而降低1 log（10）在DNA免疫中。在HLA2.1转基因小鼠中，HCV-LP免疫导致1-2 log（10）减少，而DNA免疫未见减少。淋巴细胞从HCV-LP免疫小鼠到幼稚小鼠的过继转移提供了针对vvHCV.S攻击的保护，这种转移的免疫可以通过CD4或CD8耗竭而消除。我们的研究结果表明，HCV-LPs可以诱导体液和细胞免疫应答，在替代HCV攻击模型中具有保护作用，并且CD4和CD8效应淋巴细胞提供的强大细胞免疫对于保护免受HCV感染可能是重要的。 [参考：34]

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