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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Golgi-dependent transport of cholesterol to the Chlamydia trachomatis inclusion
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Golgi-dependent transport of cholesterol to the Chlamydia trachomatis inclusion

机译：高尔基依赖性胆固醇向沙眼衣原体包涵体的转运

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摘要

Cholesterol, a lipid not normally found in prokaryotes, was identified in purified Chlamydia trachomatis elementary bodies and in the chlamydial parasitophorous vacuole (inclusion) membrane of infected HeLa cells. Chlamydiae obtained eukaryotic host cell cholesterol both from de novo synthesis or low-density lipoprotein. Acquisition of either de novo-synthesized cholesterol or low-density lipoprotein-derived cholesterol was microtubule-dependent and brefeldin A-sensitive, indicating a requirement for the Golgi apparatus. Transport also required chlamydial protein synthesis, indicative of a pathogen-directed process. The cholesterol trafficking pathway appears to coincide with a previously characterized delivery of sphingomyelin to the inclusion in that similar pharmacological treatments inhibited transport of both sphingomyelin and cholesterol. These results support the hypothesis that sphingomyelin and cholesterol may be cotransported via a Golgi-dependent pathway and that the chlamydial inclusion receives cholesterol preferentially from a brefeldin A-sensitive pathway of cholesterol trafficking from the Golgi apparatus to the plasma membrane. [References: 52]
机译：在纯化的沙眼衣原体基本体和感染的HeLa细胞的衣原体寄生虫液泡（包含）膜中鉴定出胆固醇，这是原核生物中通常不存在的脂质。衣原体通过从头合成或低密度脂蛋白获得真核宿主细胞胆固醇。从头合成的胆固醇或低密度脂蛋白衍生的胆固醇的获取是微管依赖的和布雷菲德菌素A敏感的，表明需要高尔基体。运输还需要衣原体蛋白合成，这表明病原体指导过程。胆固醇的运输途径似乎与鞘磷脂的先前特征性递送相一致，因为相似的药理学疗法抑制了鞘磷脂和胆固醇的运输。这些结果支持以下假设：鞘磷脂和胆固醇可能通过高尔基体依赖性途径共转运，衣原体包涵体优先从布雷菲德菌素A敏感的胆固醇从高尔基体转运到质膜的途径接受胆固醇。 [参考：52]

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