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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Large-scale comparison of intron positions in mammalian genes shows intron loss but no gain
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Large-scale comparison of intron positions in mammalian genes shows intron loss but no gain

机译：大规模比较内含子在哺乳动物基因中的位置显示内含子缺失但无收获

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摘要

We compared intron-exon structures in 1,560 human-mouse orthologs and 360 mouse-rat orthologs. The origin of differences in intron positions between species was inferred by comparison with an outgroup, Fugu for human-mouse and human for mouse-rat. Among 10,020 intron positions in the human-mouse comparison, we found unequivocal evidence for five independent intron losses in the mouse lineage but no evidence for intron loss in humans or for intron gain in either lineage. Among 1,459 positions in rat-mouse comparisons, we found evidence for one loss in rat but neither loss in mouse nor gain in either lineage. In each case, the intron losses were exact, without change in the surrounding coding sequence, and involved introns that are extremely short, with an average of 200 bp, an order of magnitude shorter than the mammalian average. These results favor a model whereby introns are lost through gene conversion with intronless copies of the gene. in addition, the finding of widespread conservation of intron-exon structure, even over large evolutionary distances, suggests that comparative methods employing information about gene structures should be very successful in correctly predicting exon boundaries in genomic sequences. [References: 18]
机译：我们在1,560个人类小鼠直系同源物和360个小鼠鼠直系同源物中比较了内含子-外显子结构。通过与外群进行比较，可以推断出物种之间内含子位置的差异的起源，Fugu是人类小鼠，而人类是老鼠。在人-鼠比较中的10,020个内含子位置中，我们发现小鼠谱系中有五个独立的内含子缺失的明确证据，但没有人中或两个谱系中内含子获得的证据。在老鼠比较中的1459个位置中，我们发现有证据表明，老鼠损失了一只，但老鼠没有损失，也没有任何一种谱系增加。在每种情况下，内含子的损失都是精确的，不会改变周围的编码序列，涉及的内含子非常短，平均为200 bp，比哺乳动物的平均水平短一个数量级。这些结果有利于通过内含子通过基因转化而使内含子通过基因转化而丢失的模型。另外，发现内含子-外显子结构即使在较大的进化距离上也具有广泛的保守性，这表明采用有关基因结构信息的比较方法应能非常成功地正确预测基因组序列中的外显子边界。 [参考：18]

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