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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Rapid biosynthesis of N-linolenoyl-L-glutamine, an elicitor of plant volatiles, by membrane-associated enzyme(s) in Manduca sexta
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Rapid biosynthesis of N-linolenoyl-L-glutamine, an elicitor of plant volatiles, by membrane-associated enzyme(s) in Manduca sexta

机译：膜相关酶在满天蛾中快速生物合成N-亚麻酸-L-谷氨酰胺（一种植物挥发物的引发剂）

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摘要

In response to elicitors in the oral secretions of caterpillars, plants produce and release volatile chemicals that attract predators and parasitoids of the caterpillar while it feeds. The most prevalent elicitors are fatty acid amides consisting of 18-carbon polyunsaturated fatty acids coupled with L-glutamine. We demonstrate rapid CoA- and ATP-independent in vitro biosynthesis of the fatty acid amide elicitor, N-linolenoyl-L-glutamine, by microsomal fractions of several alimentary tissues in Manduca sexta. N-linolenoyl-L-glutamine is a structural analog of several other elicitors including volicitin, the first fatty acid amide elicitor identified in caterpillars. The enzyme(s) that catalyzed biosynthesis of N-linolenoyl-L-glutamine was localized within the integral membrane protein fraction extracted from microsomes by Triton X-114 detergent phase partitioning and had maximum activity at alkaline pH. We found no evidence suggesting microbial or tissue-independent biosynthesis of N-linolenoyl-L-glutamine in M. sexta. The in vitro biosynthesis of N-linolenoyl-L-glutamine by membrane-associated enzyme(s) in M. sexta represents direct evidence of fatty acid amide synthesis by caterpillar tissues. [References: 33]
机译：为了响应毛虫的口腔分泌物中的引发剂，植物会产生并释放挥发性化学物质，这些化学物质在它们进食时会吸引毛虫的捕食者和拟寄生物。最普遍的引发剂是由18-碳多不饱和脂肪酸与L-谷氨酰胺偶联而成的脂肪酸酰胺。我们展示了快速的CoA和ATP依赖的体外生物合成的脂肪酸酰胺引发剂，N-亚麻酸-L-谷氨酰胺，由六种曼杜卡六倍体消化组织的微粒体级分组成。 N-亚麻酸-L-谷氨酰胺是其他几种引发剂的结构类似物，包括volicitin，这是在毛毛虫中鉴定的第一个脂肪酸酰胺引发剂。催化N-亚麻酸-L-谷氨酰胺生物合成的酶位于通过Triton X-114去污剂相分配从微粒体提取的完整膜蛋白级分中，并且在碱性pH下具有最大活性。我们没有发现证据表明性分枝杆菌中N-亚麻酸-L-谷氨酰胺的微生物或组织非依赖性生物合成。羊膜支原体中膜相关酶体外生物合成N-亚麻酸-L-谷氨酰胺代表了毛虫组织合成脂肪酸酰胺的直接证据。 [参考：33]

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