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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Control of in vitro organogenesis by cyclin-dependent kinase activities in plants
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Control of in vitro organogenesis by cyclin-dependent kinase activities in plants

机译：通过细胞周期蛋白依赖性激酶活性控制体外器官发生

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摘要

Little is known concerning the mechanisms by which auxin and cytokinin exert their effects on proliferation and differentiation. Cyclin-dependent kinases (CDKs) are major regulators of the eukaryotic cell cycle, thus they are assumed to control cell differentiation as well as proliferation in response to phytohormone signals. Here, we overexpressed rice R2 cDNA, which encodes a CDK-activating kinase, in tobacco leaf explants by using the glucocorticoid-mediated transcriptional induction system. Transient expression of R2 during the first 7 days of culture triggered callus formation in the absence of cytokinin. This phenotype was enhanced by higher expression of R2 or coexpression of cyclin H, and suppressed by treatment with roscovitine, a CDK inhibitor. R2 expression at a later stage did not prevent cells from differentiation into roots, suggesting a restricted period for sensing CDK activities that control differentiation fate of cells during organogenesis. [References: 35]
机译：关于植物生长素和细胞分裂素对增殖和分化发挥作用的机制知之甚少。细胞周期蛋白依赖性激酶（CDKs）是真核细胞周期的主要调节剂，因此被认为可控制细胞分化以及对植物激素信号的响应增殖。在这里，我们通过使用糖皮质激素介导的转录诱导系统在烟草叶外植体中过表达水稻R2 cDNA，它编码CDK激活激酶。在培养的前7天，R2的瞬时表达在没有细胞分裂素的情况下触发了愈伤组织的形成。 R2的更高表达或细胞周期蛋白H的共表达增强了该表型，而CDK抑制剂roscovitine治疗抑制了该表型。 R2在后期的表达并不能阻止细胞分化为根，这暗示了在器官发生过程中感知控制细胞分化命运的CDK活性的有限时期。 [参考：35]

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