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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus
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Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus

机译：RRS1-R（赋予抗青枯病的蛋白质）与PopP2（针对植物核的III型效应子）之间的物理相互作用

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摘要

RRS1-R confers broad-spectrum resistance to several strains of the causal agent of bacterial wilt, Ralstonia solanacearum. Although genetically defined as recessive, this R gene encodes a protein whose structure combines the TIR-NBS-LRR domains found in several R proteins and a WRKY motif characteristic of some plant transcriptional factors and behaves as a dominant gene in transgenic susceptible plants. Here we show that PopP2, a R. solanacearum type III effector, which belongs to the YopJ/AvrRxv protein family, is the avirulence protein recognized by RRS1-R. Furthermore, an interaction between PopP2 and both RRS1-R and RRS1-S, present in the resistant Nd-1 and susceptible Col-5 Arabidopsis thaliana ecotypes, respectively, was detected by using the yeast split-ubiquitin two-hybrid system. This interaction, which required the full-length R protein, was not observed between the RRS1 proteins and PopP1, another member of the YopJ/AvrRxv family present in strain GMI1000 and that confers avirulence in Petunia. We further demonstrate that both the Avr protein and the RRS1 proteins colocalize in the nucleus and that the nuclear localization of the RRS1 proteins are dependent on the presence of PopP2. [References: 41]
机译：RRS1-R对几种细菌性枯萎病青枯雷尔氏菌的致病菌菌株具有广谱抗性。尽管从基因上被定义为隐性的，但该R基因编码的蛋白质的结构结合了在几种R蛋白质中发现的TIR-NBS-LRR结构域和某些植物转录因子的WRKY基序特征，并在转基因易感植物中表现为显性基因。在这里我们显示PopP2，一种青枯菌III型效应子，属于YopJ / AvrRxv蛋白家族，是RRS1-R识别的无毒蛋白。此外，使用酵母分裂泛素双杂交系统检测了分别存在于抗性Nd-1和易感Col-5拟南芥生态型中的PopP2与RRS1-R和RRS1-S之间的相互作用。在RRS1蛋白和PopP1之间没有观察到这种相互作用，该相互作用需要全长R蛋白，而PopP1是存在于菌株GMI1000中的YopJ / AvrRxv家族的另一个成员，并且赋予矮牵牛无毒力。我们进一步证明，Avr蛋白和RRS1蛋白都在细胞核中共定位，并且RRS1蛋白的核定位取决于PopP2的存在。 [参考：41]

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