...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A newly discovered cholesteryl galactoside from Borrelia burgdorferi
【24h】

A newly discovered cholesteryl galactoside from Borrelia burgdorferi

机译：伯氏疏螺旋体中新发现的胆固醇半乳糖苷

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two major glycolipids, which comprise approximate to36% of the total lipid mass from Borrelia burgdorferi, the etiological agent of Lyme disease, were investigated. We determined the fatty acid type, sugar identity, anomeric configuration, and substituent type and position. The structures were identified as cholesteryl 6-O-acyl-beta-D-galactopyranoside (B. burgdorferi glycolipid 1, BbGL-1), and 1,2-di-O-acyl-3-O-alpha-D-galactopyranosyl-sn-glycerol (BbGL-11). The major fatty acids Were palmitate and oleate. The structures were corroborated by gas-liquid chromatography MS, matrix-assisted laser desorption/ionization time-of-flight spectroscopy, fast atom bombardment MS, detailed NMR spectrometry, and metabolic labeling. This is a previously undescribed demonstration of a cholesteryl galactoside in bacteria. Lipopolysaccharide was not detected in B. burgdorferi. The two glycolipids have several properties suggesting they may function as lipopolysaccharide: both are main components of the bacterial membrane, surface exposed, and have a three-domain structure. BbGL-1 elicited specific antibodies in mice and rabbits, and BbGL-11 elicited antibodies that reacted with both glycolipids. [References: 25]
机译：研究了两种主要糖脂，它们占莱姆病病原体伯氏疏螺旋体的总脂质质量的约36％。我们确定了脂肪酸类型，糖同一性，端基构型以及取代基类型和位置。该结构被鉴定为胆甾醇基6-O-酰基-β-D-吡喃半乳糖苷（B. burgdorferi糖脂1，BbGL-1）和1,2-二-O-酰基-3-O-α-D-吡喃半乳糖基-锡甘油（BbGL-11）。主要脂肪酸为棕榈酸酯和油酸酯。该结构通过气液色谱MS，基质辅助激光解吸/电离飞行时间光谱，快速原子轰击MS，详细的NMR光谱和代谢标记得到了证实。这是细菌中胆固醇基半乳糖苷的先前未描述的证明。在伯氏疏螺旋体中未检测到脂多糖。这两种糖脂具有多种性质，表明它们可以充当脂多糖：两者都是细菌膜的主要成分，表面暴露，并具有三结构域结构。 BbGL-1引起小鼠和兔子的特异性抗体，BbGL-11引起与两种糖脂反应的抗体。 [参考：25]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号