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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of histidine kinases that act as sensors in the perception of salt stress in Synechocystis sp. PCC 6803.
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Identification of histidine kinases that act as sensors in the perception of salt stress in Synechocystis sp. PCC 6803.

机译：组氨酸激酶的鉴定，该组氨酸激酶可用于感知集藻藻中的盐胁迫。 PCC 6803。

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摘要

In plants and microorganisms, salt stress regulates the expression of large numbers of genes. However, the machinery that senses salt stress remains to be characterized. In this study we identified sensory histidine kinases that are involved in the perception of salt stress in the cyanobacterium Synechocystis sp. strain PCC 6803. A library of strains with mutations in all 43 histidine kinases was screened by DNA microarray analysis of genomewide gene expression under salt stress. The results suggested that four histidine kinases, namely, Hik16, Hik33, Hik34, and Hik41, perceived and transduced salt signals. However, Hik33, Hik34, and Hik16 acting with Hik41 regulated the expression of different sets of genes. These histidine kinases regulated the expression of approximately 20% of the salt-inducible genes, whereas the induction of the remaining salt-inducible genes was unaffected by mutations in any of the histidine kinases, suggesting that additional sensory mechanisms might operate in the perception of salt stress. We also used DNA microarrays to investigate the effect of various salts on gene expression. Our results indicate that Hik33 responds to sodium salts and not to KCl, whereas the Hik16/Hik41 system responds only to NaCl.
机译：在植物和微生物中，盐胁迫调节大量基因的表达。但是，感知盐分胁迫的机械仍待表征。在这项研究中，我们确定了感觉组织氨酸激酶，它们参与了蓝藻Synechocystis sp。中盐胁迫的感知。菌株PCC6803。通过在盐胁迫下对全基因组基因表达进行DNA微阵列分析，筛选了所有43个组氨酸激酶均具有突变的菌株文库。结果表明，四种组氨酸激酶，即Hik16，Hik33，Hik34和Hik41，可以感知和转导盐信号。但是，与Hik41相互作用的Hik33，Hik34和Hik16调节了不同基因集的表达。这些组氨酸激酶调节约20％的盐诱导基因的表达，而其余盐诱导基因的诱导不受任何组氨酸激酶突变的影响，表明其他感觉机制可能在盐的感知中起作用强调。我们还使用DNA微阵列来研究各种盐对基因表达的影响。我们的结果表明，Hik33对钠盐而不是对KCl响应，而Hik16 / Hik41系统仅对NaCl响应。

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