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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy.
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Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy.

机译：通过相干抗斯托克斯拉曼散射显微镜观察的磷脂双层之间的水分子排列。

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摘要

We demonstrate ordered orientation of the hydration water at the surface of phospholipid bilayers by use of coherent anti-Stokes Raman scattering (CARS) microscopy, a highly sensitive vibrational imaging method recently developed. We investigated negatively charged POPS (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine) and neutral POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) multilamellar onions dispersed in deuterated dodecane. The imaging contrast based on the CARS signal from the H2O stretching vibration shows a clear dependence on the excitation field polarization. Our results provide direct experimental evidence that water molecules close to the phospholipid bilayer surface are ordered with the symmetry axis along the direction normal to the bilayer. Moreover, the amount of ordered water molecules depends on the lipid polar group. The spectral profile for the inter-lamellar water shows that the water molecules bound to the bilayer surface are less hydrogen-bonded and exhibit a highervibrational frequency than bulk water.
机译：我们通过使用相干的反斯托克斯拉曼散射（CARS）显微镜，一种最近开发的高灵敏度振动成像方法，证明了磷脂双层表面上的水合水的有序取向。我们研究了带负电荷的POPS（1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸-1-丝氨酸）和中性POPC（1-棕榈酰基-2-油酰基-sn-甘油-3磷酸胆碱）多层洋葱氘代十二烷。基于来自H2O拉伸振动的CARS信号的成像对比度显示出对激发场极化的明显依赖。我们的结果提供了直接的实验证据，表明靠近磷脂双层表面的水分子的排列方向与对称方向垂直于双层。此外，有序水分子的数量取决于脂质极性基团。层间水的光谱图表明，与双层水结合的水分子与氢的键合较少，并且振动频率高于散装水。

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