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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Molecular cloning and functional expression of the first two specific insect myosuppressin receptors.
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Molecular cloning and functional expression of the first two specific insect myosuppressin receptors.

机译：前两种特异性昆虫肌抑制素受体的分子克隆和功能表达。

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摘要

The Drosophila Genome Project database contains the sequences of two genes, CG8985 and CG13803, which are predicted to code for G protein-coupled receptors. We cloned the cDNAs corresponding to these genes and found that their gene structures had not been correctly annotated. We subsequently expressed the coding regions of the two corrected receptor genes in Chinese hamster ovary cells and found that each of them coded for a receptor that could be activated by low concentrations of Drosophila myosuppressin (EC50,4 x 10-8 M). The insect myosuppressins are decapeptides that generally inhibit insect visceral muscles. Other tested Drosophila neuropeptides did not activate the two receptors. In addition to the two Drosophila myosuppressin receptors, we identified a sequence in the genomic database from the malaria mosquito Anopheles gambiae that also very likely codes for a myosuppressin receptor. To our knowledge, this paper is the first report on the molecular identification of specific insect myosuppressin receptors.
机译：果蝇基因组计划数据库包含两个基因的序列，分别为CG8985和CG13803，它们编码为G蛋白偶联受体。我们克隆了与这些基因相对应的cDNA，发现它们的基因结构没有正确注释。随后，我们在中国仓鼠卵巢细胞中表达了两个校正后的受体基因的编码区，发现它们每个都编码了一种可被低浓度果蝇肌抑制素（EC50,4 x 10-8 M）激活的受体。昆虫肌抑制素是通常抑制昆虫内脏肌肉的十肽。其他测试的果蝇神经肽没有激活这两个受体。除了两个果蝇肌抑制素受体之外，我们从疟疾蚊子冈比亚按蚊的基因组数据库中鉴定了一个序列，该序列也很可能编码了肌抑制素受体。据我们所知，本文是关于特定昆虫肌抑制素受体分子鉴定的第一份报告。

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