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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane.
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The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane.

机译：酵母Rho1p和Pkc1p调节几丁质合酶III（Chs3p）从内部存储区到质膜的运输。

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摘要

During cell stress, Saccharomyces cerevisiae increases the synthesis of chitin and glucans to strengthen and repair the cell wall. In this study, we show that under conditions of cell stress, the steady-state localization of chitin synthase III (Chs3p) shifts from internal stores (chitosomes) to the plasma membrane (PM). This redistribution occurs rapidly and requires the activators of the cell wall stress response signaling pathway, the G protein Rho1p, and the protein kinase Pkc1p, but not the cell integrity response mitogen-activated protein kinase cascade. Furthermore, expression of activated forms of Rho1p or Pkc1p, in the absence of cell stress, is sufficient to redistribute Chs3p to the PM. In cells deficient for both the clathrin adaptor complex 1 and Chs6p, where Chs3p is transported to the PM by an alternative bypass pathway, cell wall stress did not cause mobilization of Chs3p, suggesting that Rho1p/Pkc1p regulate Chs3p exit from the trans-Golgi network. The mobilization of an intracellular reservoir of Chs3p presents a novel opportunity to investigate the genetic basis of regulated vesicular traffic.
机译：在细胞应激期间，酿酒酵母增加了几丁质和葡聚糖的合成，以增强和修复细胞壁。在这项研究中，我们表明，在细胞应激条件下，几丁质合酶III（Chs3p）的稳态定位从内部存储（壳聚糖）转移到质膜（PM）。这种重新分布迅速发生，并且需要细胞壁应激反应信号传导途径，G蛋白Rho1p和蛋白激酶Pkc1p的激活剂，但不需要细胞完整性反应丝裂原激活的蛋白激酶级联反应。此外，在没有细胞应激的情况下，Rho1p或Pkc1p激活形式的表达足以将Chs3p重新分布到PM。在缺乏网格蛋白衔接子复合物1和Chs6p的细胞中，其中Chs3p通过替代旁路途径转运至PM，细胞壁应力并未引起Chs3p的动员，这表明Rho1p / Pkc1p调节Chs3p从反高尔基网络中退出。 Chs3p的细胞内储层的动员提供了一个新的机会来调查调节的水泡交通的遗传基础。

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