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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Biofilm dispersal in Xanthomonas campestris is controlled by cell-cell signaling and is required for full virulence to plants
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Biofilm dispersal in Xanthomonas campestris is controlled by cell-cell signaling and is required for full virulence to plants

机译：生物膜在油菜黄单胞菌中的扩散受细胞信号传导的控制，对植物具有完全的毒力是必需的

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The rpf gene cluster of Xanthomonas campestris pathovar campestris (Xcc) is required for the pathogenesis of this bacterium to plants. Several rpf genes are involved in the coordinate positive regulation of the production of virulence factors mediated by the small diffusible molecule DSF (for diffusible signal factor). RpfF directs the synthesis of DSF, and a two-component sensory transduction system comprising RpfC and RpfG has been implicated in the perception of the DSF signal and signal transduction. In L medium, rpfF, rpfG, rpfC, and rpfGHC mutants grew as matrix-enclosed aggregates, whereas the wild type grew in a dispersed planktonic fashion. Synthesis of the extracellular polysaccharide xanthan was required for aggregate formation. Addition of DSF triggered dispersion of the aggregates formed by the rpfF strain, but not those of rpf strains defective in DSF signal transduction. An extracellular enzyme from Xcc whose synthesis was positively controlled by the DSF/rpf system could disperse the aggregates produced by all rpf strains. The enzyme was identified as the single endo-beta-1,4-mannanase encoded by the Xcc genome. This enzyme had no detectable activity against soluble xanthan. The endo-beta-1,4-mannanase was required for the full virulence of Xcc to plants. On the basis of this model system, we propose that one role of the beta-mannanase during disease is to promote transitions from an aggregated or biofilm lifestyle to a planktonic lifestyle in response to the DSF signal. [References: 34]
机译：Xanthomonas campestris pathovar campestris（Xcc）的rpf基因簇是该细菌向植物致病的必需条件。几个rpf基因参与由小扩散分子DSF（对于扩散信号因子）介导的毒力因子产生的协同正向调控。 RpfF指导DSF的合成，并且包含RpfC和RpfG的两组分感觉转导系统已牵涉到DSF信号和信号转导的感知中。在L培养基中，rpfF，rpfG，rpfC和rpfGHC突变体以基质封闭的聚集体形式生长，而野生型则以分散的浮游方式生长。聚集体形成需要细胞外多糖黄原胶的合成。 DSF的添加触发了由rpfF菌株形成的聚集体的分散，但没有引发DSF信号转导缺陷的rpf菌株的聚集体的分散。 Xcc的一种细胞外酶，其合成受到DSF / rpf系统的正调控，可以分散所有rpf菌株产生的聚集体。该酶被鉴定为Xcc基因组编码的单个内切β-1,4-甘露聚糖酶。该酶对可溶性黄原胶没有可检测的活性。 Xcc对植物的完全毒力需要内切β-1,4-甘露聚糖酶。在此模型系统的基础上，我们建议β-甘露聚糖酶在疾病发生过程中的一个作用是响应DSF信号促进从聚集或生物膜生活方式向浮游生活方式的转变。 [参考：34]

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