...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Functional map of human cytomegalovirus AD169 defined by global mutational analysis.
【24h】

Functional map of human cytomegalovirus AD169 defined by global mutational analysis.

机译：通过全局突变分析定义的人类巨细胞病毒AD169功能图。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human cytomegalovirus has a complex double-stranded DNA genome of approximately 240,000 bp that contains approximately 150 ORFs likely to encode proteins, most of whose functions are not well understood. We have used an infectious bacterial artificial chromosome to introduce 413 defined insertion and substitution mutations into the human cytomegalovirus AD169 genome by random and site-directed transposon mutagenesis. Mutations were produced in all unique ORFs with a high probability of encoding proteins for which mutants have not been previously documented and in many previously characterized ORFs. The growth of selected mutants was assayed in cultured human fibroblasts, and we now recognize 41 essential, 88 nonessential, and 27 augmenting ORFs. Most essential and augmenting genes are located in the central region, and nonessential genes generally cluster near the ends of the viral genome.
机译：人类巨细胞病毒具有约240,000 bp的复杂双链DNA基因组，其中包含约150个可能编码蛋白质的ORF，其大多数功能尚不为人所知。我们已使用传染性细菌人工染色体通过随机和定点转座子诱变将413种定义的插入和取代突变引入人巨细胞病毒AD169基因组。在所有独特的ORF中都产生了突变，极有可能编码先前没有文献记载的突变体的蛋白质以及在许多先前表征的ORF中产生的蛋白质。在培养的人类成纤维细胞中检测了选定突变体的生长，现在我们认识到41个必需，88个非必需和27个扩增ORF。最重要和最重要的基因位于中央区域，非必需基因通常聚集在病毒基因组末端附近。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号