Enzyme screening with synthetic multifunctional pores: Focus on biopolymers.

Sorde N; Das G; Matile S

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Enzyme screening with synthetic multifunctional pores: Focus on biopolymers.

机译：具有合成多功能孔的酶筛：专注于生物聚合物。

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This report demonstrates that a single set of identical synthetic multifunctional pores can detect the activity of many different enzymes. Enzymes catalyzing either synthesis or degradation of DNA (exonuclease III or polymerase I), RNA (RNase A), polysaccharides (heparinase I, hyaluronidase, and galactosyltransferase), and proteins (papain, ficin, elastase, subtilisin, and pronase) are selected to exemplify this key characteristic of synthetic multifunctional pore sensors. Because anionic, cationic, and neutral substrates can gain access to the interior of complementarily functionalized pores, such pores can be the basis for very user-friendly screening of a broad range of enzymes.

机译：该报告表明，一组相同的合成多功能孔可以检测许多不同酶的活性。选择可催化DNA（核酸外切酶III或聚合酶I），RNA（RNase A），多糖（肝素酶I，透明质酸酶和半乳糖基转移酶）和蛋白质（木瓜蛋白酶，粘蛋白，弹性蛋白酶，枯草杆菌蛋白酶和链霉蛋白酶）合成或降解的酶。举例说明了合成多功能孔隙传感器的关键特性。由于阴离子，阳离子和中性底物可以进入互补功能化孔的内部，因此这些孔可以成为对多种酶进行非常用户友好的筛选的基础。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2003年第21期|P.11964-11969|共6页
作者
Sorde N; Das G; Matile S;
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作者单位

Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Aspects of disease screening; Enzymes; enzymology; Biopolymers; 酶类; 生物聚合物;

机译：Aspects of disease screening;Enzymes;enzymology;Biopolymers;酶类;生物聚合物;

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1. On selectivity and sensitivity of synthetic multifunctional pores as enzyme sensors: Discrimination between ATP and ADP and comparison with biological pores [J] . Sorde N., Matile S. Biopolymers: Original Research on Biomolecules and Biomolecular Assemblies . 2004,第1期

机译：关于合成多功能孔作为酶传感器的选择性和敏感性：ATP和ADP的区别以及与生物孔的比较
2. Enzyme engineering: Optimized enzymes by means of focused libraries and cluster screening [J] . Greiner-St?ffele T. Biospektrum . 2012,第4期

机译：酶工程：通过聚焦文库和簇筛选优化酶
3. Novel synthetic routes of large-pore magnetic mesoporous nanocomposites (SBA-15/Fe3O4) as potential multifunctional theranostic nanodevices [J] . Vargas-Osorio Z., Gonzalez-Gomez M. A., Pineiro Y., Journal of Materials Chemistry, B. materials for biology and medicine . 2017,第47期

机译：大孔磁性介孔纳米复合材料（SBA-15 / FE3O4）的新型合成路线作为潜在的多功能治疗纳米型
4. High-throughput screening methods for directed enzyme evolution:Focus on Microbial display [C] . China-Japan-Korea Joint Symposium on Enzyme Engineering . 2004

机译：高通量筛选可指导酶进化的方法：微生物展示的重点
5. Study of enzyme evolution within the MLE subgroup focusing on characterizing member enzymes for the purpose of discovering relationships among sequence, structure, and function. [D] . Sakai, Ayano. 2009

机译：为了发现序列，结构和功能之间的关系，MLE亚组内酶进化的研究侧重于表征成员酶。
6. Enzyme screening with synthetic multifunctional pores: Focus on biopolymers [O] . Nathalie Sordé, Gopal Das, Stefan Matile 2003

机译：具有合成多功能孔的酶筛：专注于生物聚合物
7. Enzyme screening with synthetic multifunctional pores: Focus on biopolymers [O] . Sorde, Nathalie, Das, Gopal, Matile, Stefan 2003

机译：具有合成多功能孔的酶筛：专注于生物聚合物

Enzyme screening with synthetic multifunctional pores: Focus on biopolymers.

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