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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Pannexins, a family of gap junction proteins expressed in brain
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Pannexins, a family of gap junction proteins expressed in brain

机译：Pannexins，一种在大脑中表达的间隙连接蛋白家族

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摘要

Database search has led to the identification of a family of proteins, the pannexins, which share some structural features with the gap junction forming proteins of invertebrates and vertebrates. The function of these proteins has remained unclear so far. To test the possibility that pannexins underlie electrical communication in the brain, we have investigated their tissue distribution and functional properties. Here, we show that two of these genes, pannexin 1 (Px1) and Px2, are abundantly expressed in the CNS. In many neuronal cell populations, including hippocampus, olfactory bulb, cortex and cerebellum, there is coexpression of both pannexins, whereas in other brain regions, e.g., white matter, only Px1-positive cells were found. On expression in Xenopus oocytes, Px1, but not Px2 forms functional hemichannels. Coinjection of both pannexin RNAs results in hemichannels with functional properties that are different from those formed by Px1 only. In paired oocytes, Px1, alone and in combination with Px2, induces the formation of intercellular channels. The functional characteristics of homomeric Px1 versus heteromeric Px1/Px2 channels and the different expression patterns of Px1 and Px2 in the brain indicate that pannexins form cell type-specific gap junctions with distinct properties that may subserve different functions.
机译：数据库搜索已导致鉴定出一个蛋白质家族，即pannexins，它们与无脊椎动物和脊椎动物的间隙连接形成蛋白具有某些结构特征。到目前为止，这些蛋白质的功能尚不清楚。为了测试pannexin在大脑中进行电通信的可能性，我们研究了它们的组织分布和功能特性。在这里，我们显示了其中两个基因，pannexin 1（Px1）和Px2，在CNS中大量表达。在包括海马，嗅球，皮层和小脑在内的许多神经元细胞群体中，两种pannexins都是共表达的，而在其他脑区，例如白质中，仅发现Px1阳性细胞。在非洲爪蟾卵母细胞中表达时，Px1而非Px2形成功能性半通道。两个pannexin RNA的共同注射导致半通道的功能特性与仅由Px1形成的半通道不同。在成对的卵母细胞中，单独的Px1和与Px2结合的Px1诱导细胞间通道的形成。同源Px1与异源Px1 / Px2通道的功能特征以及Px1和Px​​2在大脑中的不同表达模式表明，pannexins形成具有不同特性的细胞类型特异性间隙连接，可以发挥不同的功能。

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