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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors
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Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors

机译：人胚胎干细胞和人多能生殖细胞肿瘤中的基因表达模式

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摘要

Remarkably little is known about the transcriptional profiles of human embryonic stem (ES) cells or the molecular mechanisms that underlie their pluripotency. To identify commonalties among the transcriptional profiles of different human pluripotent cells and to search for clues into the genesis of human germ cell tumors, we compared the expression profiles of human ES cell lines, human germ cell tumor cell lines and tumor samples, somatic cell lines, and testicular tissue samples by using cDNA microarray analysis. Hierarchical cluster analysis of gene expression profiles showed that the five independent human ES cell lines clustered tightly together, reflecting highly similar expression profiles. The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples. We identified 895 genes that were expressed at significantly greater levels in human ES and embryonal carcinoma cell lines than in control samples. These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype.
机译：关于人类胚胎干（ES）细胞的转录图谱或构成其多能性的分子机制知之甚少。为了确定不同人类多能细胞转录谱之间的共性并寻找人类生殖细胞肿瘤发生的线索，我们比较了人类ES细胞系，人类生殖细胞肿瘤细胞系和肿瘤样品，体细胞系的表达谱，以及通过使用cDNA微阵列分析的睾丸组织样本。基因表达谱的层次聚类分析表明，五个独立的人类ES细胞系紧密聚集在一起，反映出高度相似的表达谱。人类ES细胞系的基因表达模式与人类胚胎癌细胞样品显示出许多相似性，而与精原细胞样品则相距甚远。我们确定了895个基因，它们在人ES和胚胎癌细胞系中的表达水平明显高于对照样品。这些基因是参与维持多能，未分化表型的候选基因。

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