...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Rab27b is associated with fusiform vesicles and may be involved in targeting uroplakins to urothelial apical membranes
【24h】

Rab27b is associated with fusiform vesicles and may be involved in targeting uroplakins to urothelial apical membranes

机译：Rab27b与梭状囊泡有关，可能与将尿激酶素靶向尿路上皮根尖膜有关

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The terminally differentiated umbrella cells of bladder epithelium contain unique cytoplasmic organelles, the fusiform vesicles, which deliver preassembled crystalline arrays of uroplakin proteins to the apical cell surface of urothelial umbrella cells. We have investigated the possible role of Rab proteins in this delivery process, and found Rab27b to be expressed at an extraordinary high level (0.1 % of total protein) in urothelium, whereas Rab27b levels were greatly reduced (to <5% of normal urothelium) in cultured urothelial cells, which synthesized only small amounts of uroplakins and failed to form fusiform vesicles. Immuno-electron microscopy showed that Rab27b was associated with the cytoplasmic face of the fusiform vesicles, but not with that of the apical plasma membrane. The association of Rab27b with fusiform vesicles and its differentiation-dependent expression suggest that this Rab protein plays a role in regulating the delivery of fusiform vesicles to the apical plasma membrane of umbrella cells.
机译：膀胱上皮的终末分化伞状细胞含有独特的胞质细胞器，梭形囊泡，将预先组装的尿激酶素蛋白晶体阵列传递至尿路上皮伞状细胞的顶端细胞表面。我们研究了Rab蛋白在此递送过程中的可能作用，并发现Rab27b在尿路上皮中的表达水平非常高（占总蛋白的0.1％），而Rab27b的水平则大大降低（降至正常尿路上皮的5％）在培养的尿道上皮细胞中，其仅合成少量的尿道素并未能形成梭形囊泡。免疫电子显微镜检查显示，Rab27b与梭状囊泡的胞质面有关，但与顶质膜的胞质面无关。 Rab27b与梭形囊泡的关联及其分化依赖性表达表明，该Rab蛋白在调节梭形囊泡向伞状细胞顶质膜的传递中发挥作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号