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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genetic and functional differences between multipotent neural and pluripotent embryonic stem cells
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Genetic and functional differences between multipotent neural and pluripotent embryonic stem cells

机译：多能神经和多能胚胎干细胞之间的遗传和功能差异

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摘要

Stem cells (SCs) are functionally defined by their abilities to self-renew and generate differentiated cells. Although much effort has been focused on defining the common characteristics among various types of SCs, the genetic and functional differences between multipotent and pluripotent SCs have garnered less attention. We report a direct genetic and functional comparison of molecularly defined and clonally related populations of neural SCs (NSCs) and embryonic SCs (ESCs), using the Sox2 promoter for isolation of purified populations by fluorescence-activated cell sorting. A stringent expression profile comparison of promoter-defined NSCs and ESCs revealed a striking dissimilarity, and subsequent chimera analyses confirmed the fundamental differences in cellular potency between these populations. This direct comparison elucidates the molecular basis for the functional differences in pluripotent ESCs and multipotent NSCs.
机译：干细胞（SC）在功能上由其自我更新和生成分化细胞的能力定义。尽管已经集中精力来定义各种类型的SC之间的共同特征，但是多能SC和多能SC之间的遗传和功能差异却很少受到关注。我们报告了直接的遗传和功能比较的分子定义和克隆相关的神经SC（NSCs）和胚胎SC（ESCs）的人口，使用Sox2启动子通过荧光激活细胞分选分离纯化的人口。启动子定义的NSC和ESC的严格表达谱比较显示出惊人的差异，随后的嵌合体分析证实了这些人群之间细胞效能的根本差异。这种直接比较阐明了多能ESC和多能NSC中功能差异的分子基础。

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