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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response
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The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response

机译：烟草水杨酸结合蛋白3（SABP3）是叶绿体碳酸酐酶，具有抗氧化活性，并在超敏防御反应中起作用

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摘要

In plants, salicylic acid (SA) plays an important role in signaling both local and systemic defense responses. Previous efforts to identify SA effector proteins in tobacco have led to the isolation of two soluble cytoplasmic SA-binding proteins (SABPs): catalase, SABP, and an ≈25-kDa protein, SABP2. Here we describe the identification of an SA-binding protein, SABP3, in the stroma of tobacco chloroplasts. SABP3 bound SA with an apparent dissociation constant (K_d) of 3.7 /μM and exhibited much greater affinity for biologically active than inactive analogs. Purification and partial sequencing of SABP3 indicated that it is the chloroplast carbonic anhydrase (CA). Confirming this finding, recombinant tobacco chloroplast CA exhibited both CA enzymatic and SA-binding activities. Expression of this protein in yeast also demonstrated that CA/SABP3 has antioxidant activity. A second gene encoding CA was also cloned, and its encoded protein was shown to behave similarly to that purified as SABP3. Finally, silencing of CA gene expression in leaves suppressed the Pto:avrPto-mediated hypersensitive response in disease resistance. These results demonstrate that SA may act through multiple effector proteins in plants and shed further light on the function of CA in chloroplasts.
机译：在植物中，水杨酸（SA）在信号传递局部和全身防御反应中均起重要作用。以前在烟草中鉴定SA效应蛋白的工作已导致分离出两种可溶性细胞质SA结合蛋白（SABP）：过氧化氢酶SABP和≈25-kDa蛋白SABP2。在这里，我们描述了烟草叶绿体基质中SA结合蛋白SABP3的鉴定。 SABP3结合SA的表观解离常数（K_d）为3.7 /μM，并且对生物活性的亲和力比无活性的类似物高得多。 SABP3的纯化和部分测序表明它是叶绿体碳酸酐酶（CA）。证实这一发现的重组烟草叶绿体CA展示了CA酶和SA结合活性。该蛋白质在酵母中的表达还证明CA / SABP3具有抗氧化活性。还克隆了第二个编码CA的基因，其编码的蛋白质表现出与纯化为SABP3相似的蛋白质。最后，叶片中CA基因表达的沉默抑制了Pto：avrPto介导的抗病性超敏反应。这些结果表明SA可能通过植物中的多种效应蛋白起作用，并进一步阐明了CA在叶绿体中的功能。

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