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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Stimulation of the cell cycle and maize transformation by disruption of the plant retinoblastoma pathway
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Stimulation of the cell cycle and maize transformation by disruption of the plant retinoblastoma pathway

机译：通过破坏植物视网膜母细胞瘤途径刺激细胞周期和玉米转化

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摘要

The genome of the Mastreviruses encodes a replication-associated protein (RepA) that interacts with members of the plant retino-blastoma-related protein family, which are putative cell cycle regulators. Expression of ZmRb1, a maize retinoblastoma-related gene, and RepA inhibited and stimulated, respectively, cell division in tobacco cell cultures. The effect of RepA was mitigated by over-expression of ZmRb1. RepA increased transformation frequency and callus growth rate of high type II maize germplasm. RepA-containing transgenic maize calli remained embryogenic, were readily regenerable, and produced fertile plants that transmitted transgene expression in a Mendelian fashion. In high type II, transformation frequency increased with the strength of the promoter driving RepA expression. When a construct in which RepA was expressed behind its native LIR promoter was used, primary transformation frequencies did not improve for two elite Pioneer maize inbreds. However, when LIR:RepA-containing transgenic embryos were used in subsequent rounds of transformation, frequencies were higher in the RepA+ embryos. These data demonstrate that RepA can stimulate cell division and callus growth in culture, and improve maize transformation.
机译：Mastreviruses的基因组编码一个复制相关蛋白（RepA），该蛋白与植物视网膜母细胞瘤相关蛋白家族的成员相互作用，后者是假定的细胞周期调节剂。玉米视网膜母细胞瘤相关基因ZmRb1的表达和RepA分别抑制和刺激了烟草细胞培养物中的细胞分裂。 ZmRb1的过表达减轻了RepA的影响。 RepA提高了高II型玉米种质的转化频率和愈伤组织生长速率。含RepA的转基因玉米愈伤组织保持胚性，易于再生，并产生了以孟德尔方式传递转基因表达的可育植物。在高类型II中，转化频率随着驱动RepA表达的启动子强度的增加而增加。当使用其中在其天然LIR启动子之后表达RepA的构建体时，对于两个优良的先锋玉米自交系而言，初级转化频率没有提高。但是，当在随后的几轮转化中使用含有LIR：RepA的转基因胚胎时，RepA +胚胎的频率更高。这些数据表明，RepA可以刺激培养物中的细胞分裂和愈伤组织生长，并改善玉米转化。

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