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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Histone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast
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Histone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast

机译：组蛋白H3赖氨酸4甲基化由Set1介导，并促进裂变酵母中活性染色质状态的维持

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摘要

Methylation of histone H3 at lysine 4 (H3 Lys-4) or lysine 9 (H3 Lys-9) is known to define active and silent chromosomal domains respectively from fission yeast to humans. However, in budding yeast, H3 Lys-4 methylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA. Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 Lys-4 methylation in fission yeast. Unlike in budding yeast, Setl-mediated H3 Lys-4 methylation is not required for heterochromatin assembly at the silent mating-type region and centromeres in fission yeast. Our analysis suggests that H3 Lys-4 methylation is a stable histone modification present throughout the cell cycle, including mitosis. The loss of H3 Lys-4 methylation in set1Δ cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys-4 methylation and acetylation of the H3 tail. We suggest that methylation of H3 Lys-4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys-9 methylation.
机译：已知组蛋白H3在赖氨酸4（H3 Lys-4）或赖氨酸9（H3 Lys-9）处的甲基化分别定义了从裂变酵母到人类的活性和沉默染色体结构域。但是，在发芽酵母中，H3 Lys-4甲基化对于端粒和核糖体DNA的沉默染色质组装也是必需的。在这里，我们证明了set1的缺失消除了裂变酵母中的H3 Lys-4甲基化，该set1编码一种蛋白质，该蛋白质在其氨基末端包含一个RNA识别基序，在羧基末端包含一个SET域。与发芽酵母不同，在裂变酵母的沉默交配型区域和着丝粒的异染色质装配中，不需要Set1介导的H3 Lys-4甲基化。我们的分析表明，H3 Lys-4甲基化是整个细胞周期（包括有丝分裂）中存在的稳定的组蛋白修饰。 set1Δ细胞中H3 Lys-4甲基化的丧失与组蛋白H3乙酰化水平的降低相关，表明H3 Lys-4甲基化与H3尾巴的乙酰化之间存在机械联系。我们建议，H3 Lys-4的甲基化主要在维持转录平衡的常染色体结构域中起作用，并且这种修饰对于裂殖酵母中异染色质的形成是可有可无的，而裂变酵母则利用H3 Lys-9甲基化。

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