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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Glutamic and aminoadipic semialdehydes are the main carbonyl products of metal-catalyzed oxidation of proteins
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Glutamic and aminoadipic semialdehydes are the main carbonyl products of metal-catalyzed oxidation of proteins

机译：谷氨酸和氨基己二半醛是金属催化蛋白质氧化的主要羰基产物

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Metal-catalyzed oxidation results in loss of function and structural alteration of proteins. The oxidative process affects a variety of side amino acid groups. some of which are converted to carbonyl compounds. Spectrophotometric measurement of these moieties, after their reaction with 2,4-dinitrophenylhydrazine. is a simple, accurate technique that has been widely used to reveal increased levels of protein carbonyls in aging and disease. We have initiated studies aimed at elucidating the chemical nature of protein car- bonyls. Methods based on gas chromatography/mass spectrome- try with isotopic dilution were developed for the quantitation of glutamic and aminoadipic semialdehydes after their reduction to hydroxyaminovaleric and hydroxyaminocaproic acids. Analysis of model proteins oxidized in vitrO by Cu~2+/ascorbate revealed that these two compounds constitute the majority of protein carbonyls generated. Glutamic and aminoadipic semialdehydes were also detected in rat liver proteins, where they constitute ≈60% of the total protein carbonyl value. Aminoadipic semialdehyde was also measured in protein extracts from HeLa cells. and its level increased as a consequence of oxidative stress to cell cultures. These results indicate that glutamic and aminoadipic semialdehydes are the main carbonyl products of metal-catalyzed oxidation of proteins. and that this reaction is a major route leading to the generation of grot6in carbonyls in biological samples.
机译：金属催化的氧化导致蛋白质功能丧失和结构改变。氧化过程影响各种侧氨基酸。其中一些转化为羰基化合物。这些部分与2,4-二硝基苯肼反应后，用分光光度法测量。是一种简单，准确的技术，已广泛用于揭示衰老和疾病中蛋白质羰基水平的升高。我们已经开始进行研究，旨在阐明蛋白质碳的化学性质。建立了基于气相色谱/质谱联用同位素稀释的方法，用于将谷氨酸和氨基己二半醛还原为羟氨基戊酸和羟氨基己酸后进行定量。对在玻璃体中被Cu〜2 + /抗坏血酸氧化的模型蛋白的分析表明，这两种化合物构成了所生成的大部分蛋白羰基。在大鼠肝脏蛋白质中也检测到了谷氨酸和氨基己二醛，它们占总蛋白质羰基值的≈60％。还从HeLa细胞的蛋白质提取物中测量了氨基己二酸半醛。其水平由于细胞培养物的氧化应激而增加。这些结果表明，谷氨酸和氨基己二半醛是金属催化蛋白质氧化的主要羰基产物。并且该反应是导致生物样品中grot6in羰基生成的主要途径。

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