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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Preferential potentiation of fast-releasing synaptic vesicles by cAMP at the calyx of Held
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Preferential potentiation of fast-releasing synaptic vesicles by cAMP at the calyx of Held

机译：cAMP在Held的萼片处对突触小泡的优先增强作用

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摘要

We have studied the effects of cAMP on synaptic transmission at the calyx of Held and found that forskolin (an activator of ade- nylate cyclase) and 8-Br-cAMP (a membrane-permeable analog of cAMP) potentiated excitatory postsynaptic currents (EPSCs). Direct sampling of miniature EPSCs (mEPSCs) and nonstationary fluctua- tion analysis showed that mEPSCs were not modulated by cAMP, suggesting that the locus of modulation is presynaptic. Deconvo- lution was used to examine effects of cAMP on quantal-release rates. By using this method, it was shown recently that release probabilities of readily releasable vesicles are heterogeneous. Here, we show that cAMP selectively increases the number of vesicles with higher release probabilities, whereas a slow compo- nent of the EPSC. representing vesicles that fuse more slowly. is unchanged. cAMP increases the apparent Ca~2+ sensitivity for secretion, but this increase does not reflect an increase in release probability necessarily but rather an increase in the number of highly sensitive vesicles.
机译：我们研究了cAMP对Held花萼中突触传递的影响，并发现福斯高林（腺苷酸环化酶激活剂）和8-Br-cAMP（cAMP的膜可渗透性类似物）增强了兴奋性突触后电流（EPSCs）。 。对微型EPSC（mEPSC）的直接采样和非平稳波动分析表明，cEPS并未调节mEPSC，这表明调节的位点是突触前的。去卷积用于检查cAMP对定量释放速率的影响。通过使用这种方法，最近显示出易于释放的囊泡的释放概率是异质的。在这里，我们表明，cAMP选择性地增加了具有较高释放概率的囊泡的数量，而EPSC的成分则较慢。代表融合更慢的囊泡。不变。 cAMP增加了表观的Ca〜2 +分泌敏感性，但这种增加并不一定反映了释放概率的增加，而是反映了高度敏感的囊泡数量的增加。

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