...
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The Bithorax Complex of Drosophila melanogaster: Underreplication and morphology in polytene chromosomes
【24h】

The Bithorax Complex of Drosophila melanogaster: Underreplication and morphology in polytene chromosomes

机译:果蝇的Bithorax复合体:多烯染色体中的复制不足和形态。

获取原文
获取原文并翻译 | 示例
           

摘要

The level of polyteny of the Drosophila salivary gland chromo- somes was determined throughout the chromosome region 89E1--4, the locus of the Bithorax complex. A zone of underrepli- cation spans the 300 kb of DNA from the Ubx to Abd-B loci. From the centromere proximal end of the complex, a 70-kb-long gradual decrease of polytenization starts with the Ubx transcription unit and, after a floor corresponding to the abd-A locus, raises gradu- ally back to the maximum over 70 kb in the region of the Abd-B transcription unit. In flies carrying the mutation Suppressor of DNA Underreplication [Su(UR)ES]. the underreplication of the Bithorax complex is fully Suppressed. In the wild type, the Bithorax Complex forms a weak point featuring thinner bands separated by clefts or constrictions. In Su(UR)ES strain in contrast, the 89E1--4 band looks like a single solid band consisting of homogenous dense materiaI. We speculate that the wild-type Su(UR)ES protein hampers DNA replication of silenced domains and leads to their underreplication in salivary gland polytene chromosomes.
机译:果蝇唾液腺染色体的多态性水平在整个染色体区域89E1--4(Bithorax复合体的基因座)中确定。从Ubx到Abd-B位点,复制区不足300 kb。从复合体的着丝粒近端开始,Ubx转录单位开始逐渐减少70kb长的多态化,并且在对应于abd-A基因座的最低限度后逐渐增加到70kb以上的最大值。 Abd-B转录单位的区域。在携带突变的DNA抑制子[Su(UR)ES]的果蝇中。完全抑制了Bithorax复合体的复制不足。在野生型中,Bithorax Complex形成了一个薄弱点,其特征是由裂缝或缩窄分开的较薄带。相比之下,在Su(UR)ES菌株中,89E1--4条带看起来像一条由均质致密材料组成的单一实心带。我们推测,野生型Su(UR)ES蛋白会阻碍沉默结构域的DNA复制,并导致它们在唾液腺多烯染色体中的复制不足。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号