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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene Pto
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Ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene Pto

机译：番茄抗病基因Pto赋予病原体识别特异性的远古起源

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摘要

We have investigated the origin of the Pto disease resistance (R) gene that was previously identified in the wild tomato species Lycopersicon pimpinelliyolium and isolated by map-based cloning. Pto encodes a serine-threonine protein kinase that specifically recagnizes strains of Pseudomonas syringae pv. tomato (Pst) that express the avirulence gene avrPto. We examined an accessian of the distantly related wild species Lycopersicon hirsutum var. gla- bratum that exhibits avrPto-specific resistance to Pst. The Pst resistance of L. hirsutum was introgressed into a susceptible Lycopersicon esculentum background to create the near-isogenic line 96T133-3. Resistance to Pst(avrPto) in 96T133-3 was inherited as a single dominant locus and cosegregated with a restriction fragment length polymorphism detected by the Pto gene. This observation suggested that a member of the Pto gene family confers Pst(avrPto) resistance in this L. hirsutum line. Here we report the cloning and characterization of faur members of the Pto family from 96T133-3. One gene (Lhirpto) is 97/100 identical to Pto and encodes a catalytically active protein kinase that elicits a hypersensitive response when coexpressed with avrPto in leaves of Nicotiana benthamiana. In common with the Pto kinase, the Lhirpto protein Physically interacts with AvrPto and downstream members of the Pto signaling pathway. Our studies indicate that R genes of the protein kinase class may not evolve rapidly in response to pathogen pressure and rather that their ability to recognize specific Avr proteins can be highly conserved.
机译：我们已经研究了Pto抗病性（R）基因的起源，该基因先前在野生番茄物种Lycopersicon pimpinelliyolium中得到鉴定，并通过基于图的克隆进行了分离。 Pto编码一种丝氨酸-苏氨酸蛋白激酶，可特异性识别丁香假单胞菌pv株。表达无毒力基因avrPto的番茄（Pst）。我们检查了远缘野生物种Lycopersicon hirsutum var的访问者。表现出对Pst的avrPto特异抗性的舌苔。 hirsutum的Pst抗性被渗入易感的番茄，形成近等基因系96T133-3。 96T133-3对Pst（avrPto）的抗性被继承为一个显性位点，并与Pto基因检测到的限制性片段长度多态性共分离。该观察结果表明Pto基因家族的一个成员赋予该L.hirsutum品系Pst（avrPto）抗性。在这里，我们报道了来自96T133-3的Pto家族的faur成员的克隆和鉴定。一个基因（Lhirpto）与Pto同源性为97/100，并编码一种催化活性蛋白激酶，当与avrPto共表达于本氏烟草叶片中时会引起超敏反应。与Pto激酶相同，Lhirpto蛋白与AvrPto和Pto信号传导途径的下游成员发生物理相互作用。我们的研究表明，蛋白激酶类的R基因可能不会响应病原体压力而快速进化，而是可以高度保守其识别特定Avr蛋白的能力。

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