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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Alkaline-mediated differential interaction (AMDI): A simple automatable single-nucleotide polymorphism assay
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Alkaline-mediated differential interaction (AMDI): A simple automatable single-nucleotide polymorphism assay

机译：碱性介导的微分相互作用（AMDI）：一种简单的可自动化的单核苷酸多态性分析

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摘要

The key requirements for high-throughput single-nucleotide poly- morphism (SNP) typing of DNA samples in large-scale disease case-control studies are automatability. simplicity, and robustness, coupled with minimal cost. In this paper we describe a fluorescence technique for the detection of SNPs that have been amplified by using the amplification refractory mutation system (ARMS)-PCR procedure. Its performance was evaluated using 32 sequence- specific primer mixes to assign the HLA-DRB alleles to 80 lympho- blastoid cell line DNAs chosen from our database for their diversity. All had been typed previously by alternative methods, either direct sequencing or gel electrophoresis. We believe the detection system that we call AMDI (alkaline-mediated differential interaction) sat- isfies the above criteria and is suitable for general high-throughput SNP typing.
机译：在大规模疾病病例对照研究中，对DNA样品进行高通量单核苷酸多态性（SNP）分型的关键要求是自动化。简单，坚固，成本最低。在本文中，我们描述了一种荧光技术，用于检测通过使用扩增难治性突变系统（ARMS）-PCR程序扩增的SNP。使用32种序列特异性引物混合物对HLA-DRB等位基因分配给从我们数据库中选择的80种淋巴母细胞样细胞系DNA进行了评估，以评估其多样性。之前都已通过其他方法（直接测序或凝胶电泳）进行了分型。我们认为，我们称为AMDI（碱性介导的微分相互作用）的检测系统满足上述标准，适用于一般的高通量SNP分型。

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