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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >TRM1, a YY1-like suppressor of rbcS-m3 expression in maize mesophyll cells
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TRM1, a YY1-like suppressor of rbcS-m3 expression in maize mesophyll cells

机译：TRM1，一种类似YY1的玉米叶肉细胞rbcS-m3表达抑制剂

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摘要

The genes rbcS and rbcL encode, respectively, the small and large subunits of the photosynthetic carbon dioxide fixation enzyme ribulose bisphosphate carboxylase/oxygenase. There is a single rbcL gene in each chloroplast chromosome, a family of rbcS genes is located in the nuclear genome. These two genes are not ex- pressed in mesophyll cells but are in adjacent bundle-sheath cells of leaves of the C4 plant Zea mays. Two regions of the maize gene rbcS-m3 are required for suppressing expression in mesophyll cells. one region is just beyond the translation termination site in the 3’ region. and the other is several hundred base pairs upstream of the transcription start site. A binding site for a protein with limited homology to the viral, yeast, and mammalian transcription repres- sor-activator YY1 (Yin-Yang I), has now been identified in the 3' region. A maize gene for a protein with zinc fingers homolagous to those of YY1 has been isolated, characterized, and expressed in Escherichia coli. The gene is designated trm1 (transcription repres- sor-maize 1). The protein TRM1 binds to the YY1-like site and, in additian, TRM1 binds to two sequence regions in the 5’ region of the gene that have no homology to the YY1 site. Mutagenesis or deletion of any of these three sequenes eliminates repression of rbcS-m3 reporter genes in mesophyll cells.
机译：rbcS和rbcL基因分别编码光合作用二氧化碳固定酶核糖双磷酸羧化酶/加氧酶的小亚基和大亚基。每个叶绿体染色体中只有一个rbcL基因，一个rbcS基因家族位于核基因组中。这两个基因不在叶肉细胞中表达，但在C4植物玉米的叶的相邻束鞘细胞中表达。玉米基因rbcS-m3的两个区域是抑制叶肉细胞中表达所必需的。一个地区就位于3'地区的翻译终止站点之外。另一个是转录起始位点上游的数百个碱基对。现在已经在3'区域发现了与病毒，酵母和哺乳动物转录表达激活因子YY1（Yin-Yang I）同源性有限的蛋白质的结合位点。具有锌指的蛋白质的玉米基因与YY1的玉米基因已经分离，鉴定并在大肠杆菌中表达。该基因称为trm1（转录代表基因1）。蛋白TRM1结合到YY1样位点，并且在additian中，TRM1结合到基因的5'区域中与YY1位点没有同源性的两个序列区域。这三个后代中的任何一个的诱变或缺失消除了叶肉细胞中rbcS-m3报告基因的抑制。

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