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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A tumor necrosis factor α- and interleukin 6-inducible protein that interacts with the small subunit of DNA polymerase δ and proliferating cell nuclear antigen
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A tumor necrosis factor α- and interleukin 6-inducible protein that interacts with the small subunit of DNA polymerase δ and proliferating cell nuclear antigen

机译：肿瘤坏死因子α和白介素6诱导蛋白，可与DNA聚合酶δ的小亚基相互作用并增殖细胞核抗原

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摘要

A cDNA encoding a protein of 36 kDa, polymerase delta-interacting protein 1 (PDIP1), that interacts with the small subunit (p50) of DNA polymerase δ (pol δ) was identified in a two-hybrid screen of a HepG2 cDNA library by using p50 as bait. The interaction of PDIP1 with p50 was confirmed by pull-down assays, and a similar assay was used to demonstrate that PDIP1 interacts directly with the proliferating cell nuclear antigen (PCNA). PCNA and p50 bound to PDIP1 simultaneously, and PDIP1 stimulated pol δ activity in vitro in the presence, but not the absence, of PCNA, suggesting that PDIP1 also interacts functionally with both p50 and PCNA.
机译：在HepG2 cDNA文库的两杂交筛选中，鉴定了编码36 kDa的蛋白质，聚合酶δ相互作用蛋白1（PDIP1）与DNA聚合酶δ（polδ）的小亚基（p50）相互作用。用p50作为诱饵。 PDIP1与p50的相互作用已通过下拉测定法进行了确认，并且使用类似的测定法来证明PDIP1与增殖细胞核抗原（PCNA）直接相互作用。 PCNA和p50同时与PDIP1结合，并且在存在PCNA（但不存在PCNA）的情况下，PDIP1在体外刺激polδ活性，这表明PDIP1在功能上也与p50和PCNA相互作用。

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