The expression of human α-like globin genes in transgenic mice mediated by bacterial artificial chromosome

Dong-Xiao Feng; De-Pei Liu; Yue Huang

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The expression of human α-like globin genes in transgenic mice mediated by bacterial artificial chromosome

机译：人α样球蛋白基因在细菌人工染色体介导的转基因小鼠中的表达

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After screening a bacterial artificial chromosome of human genomic DNA library with human HS-40, ζ-, α-, and θ-globin probes, a 110-kb clone bearing the whole human α-globin gene cluster was obtained and rare restriction endonuclease mapping was performed. The bacterial artificial chromosome DNA was isolated, and transgenic mice were generated. Three founders were detected from 35 newborn mice. The copy numbers were 1, 2, and 2, and the expression of human α-globin genes in various tissues at different developmental stages in the transgenic mice was assayed. The human α-globin mRNA can be detected in bone marrow, kidney, liver, brain, but not in muscle, testis, or thymus.

机译：用人类HS-40，ζ-，α-和θ-globin探针筛选人类基因组DNA文库的细菌人工染色体后，获得了一个包含整个人α-globin基因簇的110 kb克隆，并进行了罕见的限制性内切核酸酶定位被执行了。分离细菌人工染色体DNA，并产生转基因小鼠。从35只新生小鼠中发现了三位创始人。拷贝数为1、2和2，并测定了人α-珠蛋白基因在转基因小鼠中处于不同发育阶段的各种组织中的表达。人α-珠蛋白mRNA可以在骨髓，肾脏，肝脏，大脑中检测到，但在肌肉，睾丸或胸腺中则不能检测到。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第26期|p.15073-15077|共5页
作者
Dong-Xiao Feng; De-Pei Liu; Yue Huang;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Effect of deletion of 5'HS3 or 5'HS2 of the human β-globin locus control region on the developmental regulation of globin gene expression in β-globin locus yeast artificial chromosome transgenic mice [J] . Kenneth R. Peterson, Christopher H. Clegg, Patrick A. Navas Proceedings of the National Academy of Sciences of the United States of America . 1996,第13期

机译：人β-球蛋白基因座控制区5'HS3或5'HS2缺失对β-球蛋白基因座酵母人工染色体转基因小鼠中球蛋白基因表达发育调控的影响
2. Mutation of gene-proximal regulatory elements disrupts human ε-, γ-, and β-globin expression in yeast artificial chromosome transgenic mice [J] . Qinghui Liu, Jorg Bungert, James Douglas Engel Proceedings of the National Academy of Sciences of the United States of America . 1997,第1期

机译：基因近端调控元件的突变破坏酵母人工染色体转基因小鼠中人ε，γ和β球蛋白的表达
3. BP1 motif in the human beta-globin promoter affects beta-globin expression during embryonic/fetal erythropoiesis in transgenic mice bearing the human beta-globin gene. [J] . Zoueva OP, Garrett LJ, Bodine D, Blood cells, molecules and diseases . 2008,第3期

机译：人β-珠蛋白启动子中的BP1基序会在携带人β-珠蛋白基因的转基因小鼠的胚胎/胎儿红细胞生成过程中影响β-珠蛋白的表达。
4. High throughput design of Bacterial Artificial Chromosomes (BACs) for cell type specific gene expression in transgenic mice [C] . David Balya, Botond Roska IEEE Biomedical Circuits and Systems Conference . 2006

机译：转基因小鼠细胞类型特异性基因表达的细菌人工染色体（BACS）的高通量设计
5. Expression of human polypeptide hormones in transgenic and transkaryotic mice: Implications for the treatment of human genetic disease. [D] . Selden, Richard F. 1986

机译：人类多肽激素在转基因和转核小鼠中的表达：对人类遗传疾病的治疗意义。
6. The expression of human α-like globin genes in transgenic mice mediated by bacterial artificial chromosome [O] . Dong-Xiao Feng, De-Pei Liu, Yue Huang, 2001

机译：人α样球蛋白基因在转基因小鼠中的表达由细菌人工染色体介导
7. The expression of human α-like globin genes in transgenic mice mediated by bacterial artificial chromosome [O] . Feng, Dong-Xiao, Liu, De-Pei, Huang, Yue, 2001

机译：人α样球蛋白基因在转基因小鼠中的表达由细菌人工染色体介导
8. Expression of the Globin Genes and Hematopoiesis in beta-Thalassemic Mice. [R] . Popp, R. A., Bolch, S. L., Shinpock, S. G., 1988

机译：珠蛋白基因和造血细胞在β地中海贫血小鼠中的表达。

The expression of human α-like globin genes in transgenic mice mediated by bacterial artificial chromosome

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