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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Self-assembly of large, ordered lamellae from non-bilayer lipids and integral membrane groteins in vitro
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Self-assembly of large, ordered lamellae from non-bilayer lipids and integral membrane groteins in vitro

机译：从非双层脂质和完整的膜凝集素体外自组装大的有序薄片

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摘要

In many biological membranes, the major lipids are "non-bilayer lipids," which in purified form cannot be arranged in a lamellar structure. The structural and functional roles of these lipids are poorly understood. This work demonstrates that the in vitro association of the two main components of a membrane, the non-bilayer lipid monogalactosyldiacylglycerol (MGD6) and the lhlorophyll-a/b light-harvesting antenna protein of photosystem II (LHOI) of pea thylakoids, leads to the formation of large, ordered lamellar structures: (I) thin-section electron microscopy and circular dichroism spectroscopy reveal that the addition of MGDG induces the transformation of isolated, disordered macroaggregates of LHCll into stacked lamellar aggregates with a long-range chiral order of the complexes; (iI1 small-angle x-ray scattering discloses that LHCIl perturbs the structure of the pure lipid and destroys the inverted hexagonal phase: and (iii) an analysis of electron micro- graphs of negatively stained 2D crystals indicates that in MGDG- i LHCII the complexes are found in an ordered macroarray. It is E Proposed that, by limiting the space available for MGDG in the macroaggregate, LHClI inhibits formation of the inverted hexag- onal phase of lipids: in thylakoids, a spatial limitation is likely to he imposed by the high concentration of membrane-associated proteins.
机译：在许多生物膜中，主要脂质是“非双层脂质”，其以纯化形式不能以层状结构排列。这些脂质的结构和功能作用了解甚少。这项工作表明，膜的两个主要成分，非双层脂质单半乳糖基二酰基甘油（MGD6）和豌豆类囊体的光系统II（lHOI）的lhlorophyll-a / b光收集触角蛋白的体外缔合可导致大的，有序的层状结构的形成：（I）薄层电子显微镜和圆二色光谱显示，MGDG的添加诱导LHC11的分离的，无序的大聚集体转变为堆积的层状聚集体，具有长距离的手性顺序。配合物; （i1小角度x射线散射揭示LHC11干扰了纯脂质的结构并破坏了倒置的六方相：和（iii）负染色2D晶体的电子显微照片分析表明，在MGDG-i LHCII中，有人提出，通过限制大聚集体中MGDG的可用空间，LHClI可以抑制脂质的倒置六相形成：在类囊体中，空间限制很可能是由高浓度的膜相关蛋白。

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