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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The Golgi-associated COPl-coated buds and vesicles contain β/γ -actin
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The Golgi-associated COPl-coated buds and vesicles contain β/γ -actin

机译：高尔基体相关的COP1包被的芽和囊泡含有β/γ-肌动蛋白

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摘要

It has been shown previously that the morphology and subcellular positioning of the Golgi complex is controlled by actin microfila- ments. To further characterize the association between actin mi- crofilaments and the Golgi complex. we have used the Closedum botulinum toxins C2 and C3. which specifically inhibit actin poly- merization and cause depolymerization of F-actin in intact cells by the ADP ribosylation of G-actin monomers and the Rho small GTP-binding protein, respectively. Normal rat kidney cells treated with C2 showed that disruption of the actin and the collapse of the Golgi complex occurred concomitantly. However, when cells were treated with C3, the actin disassembly was observed without any change in the organization of the Golgi complex. The absence of the involvement of Rho was further confirmed by the treatment with lysophosphatidic acid or microinjection with the constitu- tively activated form of RhoA, both of which induced the stress fiber formation without affecting the GoIgi complex. Immunogold electron microscopy in normal rat kidney cells revealed that beta- and Wactin isoforms were found in Golgi-associated COPI-coated buds and vesicles. Taken together. the results suggest that the Rho signaling pathway does not directly regulate Golgi-associated actin microfilaments, and that beta- and gamma-actins might be involved in the formation and/or transport of Golgi-derived vesicular or tu- bular intermediates.
机译：以前已经证明高尔基复合体的形态和亚细胞定位是由肌动蛋白微丝控制的。为了进一步表征肌动蛋白微丝和高尔基体之间的联系。我们已经使用了封闭肉毒杆菌毒素C2和C3。它们分别通过G-肌动蛋白单体的ADP核糖基化和Rho小GTP结合蛋白特异性抑制肌动蛋白的聚合并引起完整细胞中F-肌动蛋白的解聚。用C2处理的正常大鼠肾细胞显示肌动蛋白的破坏和高尔基体的崩溃同时发生。然而，当用C3处理细胞时，观察到肌动蛋白的分解，而高尔基体的组织没有任何变化。通过用溶血磷脂酸处理或用组成型活化形式的RhoA显微注射进一步证实了Rho的参与，这两者都诱导了应力纤维的形成而不影响GoIgi复合物。正常大鼠肾细胞中的免疫金电子显微镜显示，在高尔基体相关的COPI包被的芽和囊泡中发现了β和Wactin异构体。在一起。结果表明，Rho信号通路并不直接调节高尔基体相关的肌动蛋白微丝，β-和γ-肌动蛋白可能参与高尔基体来源的囊泡或管状中间体的形成和/或运输。

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