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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A Bacillus subtilis operon containing genes of unknown function senses tRNA~Trp charging and regulates expression of the genes of tryptophan biosynthesis
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A Bacillus subtilis operon containing genes of unknown function senses tRNA~Trp charging and regulates expression of the genes of tryptophan biosynthesis

机译：含有未知功能基因的枯草芽孢杆菌操纵子可感知tRNA〜Trp电荷并调节色氨酸生物合成基因的表达

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摘要

Strains of Bads subtilis containing a temperature-sensitive tryp- tophanyl-tRNA synthetase produce elevated levels of the trypto- phan pathway enzymes, when grown at high temperatures in the presence of excess tryptophan. This increase is because of reduced availability of the tryptophan-activated trP RNA-binding attenua- tion protein (TRAP). To test the hypothesis that this elevated trP gene expression was caused by the overproduction of a transcript capable of binding and sequestering TRAP. a computer program was designed to search the B. subtilis genome sequence for additional potential TRAP binding sites. A region containing a stretch of (G/A)AG trinucleotide repeats, characteristic of a TRAP binding site. was identified in the yCZAyCbK operon. We show that transcriptional regulation of the yCZAwtK operon is controlled by the T-box antitermination mechanism in response to the level of uncharged tRNATrp. and that the presence of a trPS1 mutant allele increases production of the yCZAtwK transcript. Elevated yCZA- yCbK expression was shown to activate transcription of the trP operon. Deletion of the yCZAtwK operon abolishes the trPS1 effect on trP gene expression. The purpose of increasing expres- sion of the genes of tryptophan biosynthesis in the trPS mutant would be to provide additional tryptophan to overcome the charged tRNATrp deficiency. Therefore. in B. Subhas. as in Esche- richia coli, transcription of the tryptophan biosynthetic genes is regulated in response to changes in the extent of charging of tRNATrp as well as the availability of tryptophan.
机译：当在过量色氨酸存在下于高温下生长时，含有对温度敏感的胰蛋白to-tRNA合成酶的Bads枯草菌株可产生高水平的色氨酸途径酶。这种增加是由于色氨酸激活的trP RNA结合减弱蛋白（TRAP）的可用性降低。为了检验这一假说，即trP基因表达升高是由能够结合和隔离TRAP的转录本的过量产生引起的。设计了一个计算机程序来搜索枯草芽孢杆菌基因组序列，寻找其他潜在的TRAP结合位点。包含一段（G / A）AG三核苷酸重复序列的区域，具有TRAP结合位点的特征。在yCZAyCbK操纵子中被发现。我们表明，yCZAwtK操纵子的转录调控是由T-box抗终止机制响应不带电荷的tRNATrp的水平而控制的。 trPS1突变体等位基因的存在增加了yCZAtwK转录本的产生。升高的yCZA- yCbK表达可激活trP操纵子的转录。 yCZAtwK操纵子的删除消除了trPS1对trP基因表达的影响。增加trPS突变体中色氨酸生物合成基因表达的目的是提供额外的色氨酸，以克服带电的tRNATrp缺陷。因此。在B.Subhas。如在大肠杆菌中，色氨酸生物合成基因的转录受tRNATrp充电程度和色氨酸可用性变化的调节。

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