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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Serological identification of embryonic neural proteins as highly immunogenic tumor antigens in small cell lung cancer
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Serological identification of embryonic neural proteins as highly immunogenic tumor antigens in small cell lung cancer

机译：胚胎神经蛋白作为小细胞肺癌中高度免疫原性肿瘤抗原的血清学鉴定

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摘要

Serological analysis of expression cDNA libraries (SEREX) derived from two small cell lung cancer (SCLC) cell lines using pooled sera of SCLC patients led to the isolation of 14 genes. including 4 SOX group B genes (SOX1. SOX2. SOX3, and SOX21) and ZIC2. SOX group B genes and ZIC2 encode DNA-binding proteins; SOX group B proteins regulate transcription of target genes in the presence of cofactors. whereas ZlC2 is also suspected to be a transcriptional regulator. These genes are expressed at early developmental stages in the embryonic nervous system, but are down-regulated in the adult. Although 5OX2 mRNA can be detected in some adult tissues. ZIC2 is expressed only in brain and testis. and SOX1, SOX3, and SOX2f transcripts are not detectable in normal adult tissues. Of SCLC cell lines tested, 80/100 expressed ZIC2 mRNA, and SOX1, SOX2. and SOX3 expression was detected in 40/100, 50/100, and 10/100, respectively. SOX group B and ZlC2 antigens elicited serological responses in 30-40/100 of SCLC patients in this series, at titers up to 1:10s. In sera from 23 normal adults, no antibody was detected against SOX group B or ZIC2 proteins except for one individual with low-titer anti-SOX2 antibody. Seroreactivity against SOX1 and 2 was consistently higher titered than SOX3 and 21 reactivity, suggesting SOX1 and/or SOX2 as the main antigens eliciting anti-SOX responses. Although paraneoplastic neurological syn- dromes have been associated with several SCLC antigens. neuro- logical symptoms have not been observed in patients with anti- SOX or anti-ZlC2 antibodies.
机译：使用SCLC患者的合并血清对源自两个小细胞肺癌（SCLC）细胞系的表达cDNA文库（SEREX）进行血清学分析，导致分离出14个基因。包括4个SOX B组基因（SOX1，SOX2，SOX3和SOX21）和ZIC2。 SOX B组基因和ZIC2编码DNA结合蛋白。 SOX B组蛋白在辅因子存在下调节靶基因的转录。而Z1C2也被怀疑是转录调节因子。这些基因在胚胎神经系统的早期发育阶段表达，但在成年人中被下调。尽管在某些成人组织中可以检测到5OX2 mRNA。 ZIC2仅在大脑和睾丸中表达。在正常成人组织中无法检测到SOX1，SOX3和SOX2f转录本。在测试的SCLC细胞系中，80/100表达了ZIC2 mRNA，以及SOX1，SOX2。分别在40 / 100、50 / 100和10/100中检测到SOX3和SOX3的表达。 SOX B组和ZlC2抗原在该系列中的30-40 / 100 SCLC患者中引发血清学反应，滴度最高为1：10s。在来自23名正常成年人的血清中，除一名具有低滴度抗SOX2抗体的个体外，未检测到针对SOX B组或ZIC2蛋白的抗体。对SOX1和2的血清反应性始终比SOX3和21的反应性具有更高的效价，表明SOX1和/或SOX2是引起抗SOX反应的主要抗原。尽管副肿瘤神经系统症状与几种SCLC抗原有关。抗SOX或抗Z1C2抗体的患者未观察到神经症状。

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