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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Expression profiling reveals distinct sets of genes altered during induction and regression of cardiac hypertrophy
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Expression profiling reveals distinct sets of genes altered during induction and regression of cardiac hypertrophy

机译：表达谱显示心脏肥大的诱导和消退过程中改变的不同基因集

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Although cardiac hypertrophy has been the subject of intensive investigation, regression of hypertrophy has been significantly less Studied, precluding large-scale analysis of the relationship between these processes. In the present study, using pharmacological models 0f cardiac hypertrophy in mice, expression profiling was performed with fragments of more than 4,000 genes to characterize and contrast expression changes during induction and regression of hypertrophy. Administration of angiotensin Ⅱ and isoproterenol by osmotic minipump produced increases in heart weight (15 and 45/100, respet- tively) that returned to preinduction size after drug withdrawal. From multiple expression analyses of left ventricular RNA isolated at daily time-points during cardiac hypertrophy and regression, we identified rets of genes whose expression was altered at specific stages of this process. While confirming the participation of 25 genes or pathways previously shown to be altered by hypertrophy. a larger set of 30 genes was identified whose expression had not previously been associated with cardiac hypertrophy or regression. Of the 55 genes that showed reproducible changes during the time course of induc- tion and regression, 32 genes were altered only during induction, and 8 were altered only during regression. This study identified both known and novel genes whose expression is affected at different Stages of cardiac hypertrophy and regression and demonstrates that cardiac remodeling during regression utilizes a set of genes that are distinct from those used during induction of hypertrophy.
机译：尽管心脏肥大已成为深入研究的主题，但对肥大的消退的研究却少得多，这排除了对这些过程之间关系的大规模分析。在本研究中，使用小鼠0f心脏肥大的药理模型，对超过4,000个基因的片段进行了表达谱分析，以表征和对比肥大诱导和消退期间的表达变化。渗透微泵给予血管紧张素Ⅱ和异丙肾上腺素导致心脏重量增加（分别为15和45/100），在停药后恢复到诱导前的大小。从心脏肥大和消退过程中每日时间点分离的左心室RNA的多种表达分析中，我们鉴定了在此过程的特定阶段表达改变的基因的点。在确认25种基因或通路的参与之前，先前证实肥大会改变这种通路。确定了更大的30个基因集，这些基因的表达以前与心脏肥大或消退无关。在诱导和回归的过程中显示出可复制变化的55个基因中，只有32个基因在诱导过程中发生了改变，而8个仅在回归过程中发生了改变。这项研究确定了已知的和新颖的基因，它们的表达在心脏肥大和消退的不同阶段受到影响，并证明了在消退过程中的心脏重构利用了一组与诱导肥大过程中使用的不同的基因。

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