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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genomic organization of α1 and β1 subunits of the mammalian soluble guanylyl cyclase genes
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Genomic organization of α1 and β1 subunits of the mammalian soluble guanylyl cyclase genes

机译：哺乳动物可溶性鸟苷酸环化酶基因α1和β1亚基的基因组组织

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摘要

The structures of the genes encoding the α1 and β1 subunits of murine soluble guanylyl cyclase (sGC) were determined. Full-length cDNAs isolated from mouse lungs encoding the o1 (2.5 kb) and p1 (3.3 kb) subunits are presented in this report. The α1 sGC gene is approximately 26.4 kb and contains nine exons, whereas the p1 sGC gene spans 22 kb and consists of 14 exons. The positions of exon/intron boundaries and the sizes of introns for both genes are described. Comparison of mouse genomic organization with the Human Genome Database predicted the exon/intron boundaries of the human genes and revealed that human and mouse α1 and β1 sGC genes have similar structures. Both mouse genes are localized on the third chromosome. band 3E3-F1. and are separated by a fragment that is 2/100 of the chromosomal length. The 5' untranscribed regions of α1 and β1 subunit genes were subcloned into luciferase reporter constructs. and the functional analysis of promoter activity was performed in murine neuroblastoma N1E- 115 cells. Our results indicate that the 5' untranscribed regions for both genes possess independent promoter activities and, together with the data on chromosomal localization, suggest independent regulation of both genes.
机译：确定了编码鼠源可溶性鸟苷基环化酶（sGC）α1和β1亚基的基因的结构。从该小鼠肺中分离出的全长cDNA编码为o1（2.5 kb）和p1（3.3 kb）亚基。 α1sGC基因大约26.4 kb，包含9个外显子，而p1 sGC基因跨度22 kb，由14个外显子组成。描述了两个基因的外显子/内含子边界的位置和内含子的大小。小鼠基因组组织与人类基因组数据库的比较预测了人类基因的外显子/内含子边界，并揭示了人类和小鼠的α1和β1sGC基因具有相似的结构。两种小鼠基因都位于第三条染色体上。乐队3E3-F1。并被一个2/100染色体长度的片段隔开。将α1和β1亚基基因的5'非转录区亚克隆到萤光素酶报告基因构建体中。在鼠神经母细胞瘤N1E-115细胞中进行启动子活性的功能分析。我们的结果表明，这两个基因的5'非转录区均具有独立的启动子活性，并且与染色体定位数据一起提示这两个基因的独立调控。

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